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©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
Hepatic and renal effects of oral stingless bee honey in a streptozotocin-induced diabetic rat model
Suriati Mohd Nasir, Anis Farihan Ismail, Tuan Salwani Tuan Ismail, Wan Faiziah Wan Abdul Rahman, Wan Amir Nizam Wan Ahmad, Tengku Ahmad Damitri Al- Astani Tengku Din, Kuttulebbai Nainamohammed Salam Sirajudeen
Suriati Mohd Nasir, Anis Farihan Ismail, Tengku Ahmad Damitri Al- Astani Tengku Din, Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kota Bharu 16150, Kelantan, Malaysia
Tuan Salwani Tuan Ismail, Endocrinology Laboratory, Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kota Bharu 16150, Kelantan, Malaysia
Wan Faiziah Wan Abdul Rahman, Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kota Bharu 16150, Kelantan, Malaysia
Wan Amir Nizam Wan Ahmad, Biomedicine Program, School of Health Sciences, Health Campus, Universiti Sains Malaysia, Kota Bharu 16150, Kelantan, Malaysia
Kuttulebbai Nainamohammed Salam Sirajudeen, Department of Basic Medical Sciences, Kuliyyah of Medicine, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan 25200, Pahang, Malaysia
Author contributions: Mohd Nasir S and Ismail AF conducted animal study and laboratory analysis; Tuan Ismail TS and Sirajudeen KNS designed the study objective and methodology; Wan Ahmad WAN and Wan Abdul Rahman WF reviewed and validated the histological analysis; Tengku Din TADAA conducted statistical analysis and guided the animal and laboratory study; All the authors contributed to manuscript writing; Tuan Ismail TS edited and proofread the manuscripts.
Supported by Ministry of Higher Education, Malaysia for Fundamental Research Grant Scheme FRGS/1/2019/SKK06/USM/03/6, No. 291983-329281.
Institutional animal care and use committee statement: This study was approved by the Institutional Animal Care and Use Committee USM, Kubang Kerian [USM/IACUC/2019/(120) (1020)].
Conflict-of-interest statement: All the authors declare no conflict of interest in conducting this research.
Data sharing statement: No additional data is available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
https://creativecommons.org/Licenses/by-nc/4.0/ Corresponding author: Tuan Salwani Tuan Ismail, MBBS, Associate Professor, Doctor, Endocrinology Laboratory, Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kota Bharu 16150, Kelantan, Malaysia.
tusti@usm.my
Received: December 26, 2023
Peer-review started: December 26, 2023
First decision: January 11, 2024
Revised: January 24, 2024
Accepted: March 1, 2024
Article in press: March 1, 2024
Published online: March 20, 2024
Processing time: 84 Days and 8.3 Hours
ARTICLE HIGHLIGHTS
Research background
Studies have demonstrated an inverse relationship between honey consumption and the risk of diabetes and its complications. The argument about using honey to lower blood glucose and prevent hepatorenal diabetic complications however, continued.
Research motivation
Recent research has focused on exploring the biochemical and histological effects on kidney and liver of diabetic rats given oral stingless bee honey (SBH) and compared with rats given metformin (MET), combination SBH+MET, untreated and non-diabetic rats.
Research objectives
We investigated the effect of SBH administration on the serum biochemical parameters and the histological changes in streptozotocin induced diabetic rats’ kidney and liver using hematoxylin and eosin (H&E), Periodic Acid Schiff (PAS) staining.
Research methods
Sprague Dawley rats (n = 30) grouped into 5, i.e. SBH-treated, metformin-treated (MET) and SBH+metformin-treated (SBME) non-diabetic (ND) and untreated diabetic (UNT) groups. The rats were sacrificed after 12 d of treatment, and serum, kidney and liver collected from the rats were used for biochemical parameters assessment.
Research results
SBH-treated group showed reduce in hydropic changes and mild cellularity of glomerulus as compared to ND group on H&E staining, but resembled ND group on PAS staining. Liver tissues of SBH-treated groups revealed lower fatty vacuoles formation and dilatation of blood sinusoids as compared to other groups. Biochemically, glucose reduction and lowest serum MDA, AST, ALT and ALP observed in SBH-treated compared to MET, SBME and UNT groups.
Research conclusions
The oral SBH prevent diabetic complications development indicated by liver and kidney histology of SBH-treated diabetic rats. This finding offers a good perspective of honey role in managing diabetes.
Research perspectives
Longer duration of treatment might improve the biochemical parameters. Future study on honey role in diabetic patients and metabolomic analysis to profile the serum of human treated with SBH and MET is necessary to uncover the underlying mechanisms.
