Kaplan M, Ates I, Yuksel M, Ozderin Ozin Y, Alisik M, Erel O, Kayacetin E. Thiol/disulphide homeostasis in celiac disease. World J Gastrointest Pharmacol Ther 2017; 8(2): 120-126 [PMID: 28533921 DOI: 10.4292/wjgpt.v8.i2.120]
Corresponding Author of This Article
Ihsan Ates, MD, Department of Internal Medicine, Ankara Numune Training and Research Hospital, 06500 Ankara, Turkey. dr.ihsanates@hotmail.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Case Control Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastrointest Pharmacol Ther. May 6, 2017; 8(2): 120-126 Published online May 6, 2017. doi: 10.4292/wjgpt.v8.i2.120
Thiol/disulphide homeostasis in celiac disease
Mustafa Kaplan, Ihsan Ates, Mahmut Yuksel, Yasemin Ozderin Ozin, Murat Alisik, Ozcan Erel, Ertugrul Kayacetin
Mustafa Kaplan, Mahmut Yuksel, Yasemin Ozderin Ozin, Ertugrul Kayacetin, Department of Gastroenterology, Turkey Yuksek Ihtisas Training and Research Hospital, 06500 Ankara, Turkey
Ihsan Ates, Department of Internal Medicine, Ankara Numune Training and Research Hospital, 06500 Ankara, Turkey
Murat Alisik, Ozcan Erel, Department of Biochemistry, Yıldırım Beyazıt University Medical Faculty, 06500 Ankara, Turkey
Author contributions: Kaplan M and Ates I contributed equally to this work; Kaplan M, Ates I and Yuksel M collected and analyzed the data, and drafted the manuscript; Alisik M and Erel O provided analytical oversight; Ozderin Ozin Y and Kayacetin E designed and supervised the study; Kaplan M, Ates I, Alisik M and Erel O revised the manuscript for important intellectual content; Ozderin Ozin Y and Kaycetin E offered the technical or material support; Kaplan M and Ates I provided administrative support; all authors have read and approved the final version to be published.
Institutional review board statement: The study was designed around the 2013 Brazil version of the Declaration of Helsinki. It was approved by the Turkey Yuksek Ihtisas Training and Research Hospital Ethical Board Research Commission.
Informed consent statement: Written consent was taken from all participants who were included in the study.
Conflict-of-interest statement: The authors declared that there is no conflict of interest.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at dr.ihsanates@hotmail.com. No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Ihsan Ates, MD, Department of Internal Medicine, Ankara Numune Training and Research Hospital, 06500 Ankara, Turkey. dr.ihsanates@hotmail.com
Telephone: +90-312-5084675 Fax: +90-312-3569003
Received: November 30, 2016 Peer-review started: December 2, 2016 First decision: January 5, 2017 Revised: January 25, 2017 Accepted: March 12, 2017 Article in press: March 14, 2017 Published online: May 6, 2017 Processing time: 154 Days and 10.4 Hours
Abstract
AIM
To determine dynamic thiol/disulphide homeostasis in celiac disease and to examine the associate with celiac autoantibodies and gluten-free diet.
METHODS
Seventy three patients with celiac disease and 73 healthy volunteers were enrolled in the study. In both groups, thiol/disulphide homeostasis was examined with a new colorimetric method recently developed by Erel and Neselioglu.
RESULTS
In patients with celiac disease, native thiol (P = 0.027) and total thiol (P = 0.031) levels were lower, while disulphide (P < 0.001) level, disulphide/native thiol (P < 0.001) and disulphide/total thiol (P < 0.001) ratios were higher compared to the control group. In patients who do not comply with a gluten-free diet, disulphide/native thiol ratio was found higher compared to the patients who comply with the diet (P < 0.001). In patients with any autoantibody-positive, disulphide/native thiol ratio was observed higher compared to the patients with autoantibody-negative (P < 0.05). It is found that there is a negative correlation between celiac autoantibodies, and native thiol, total thiol levels and native thiol/total thiol ratio, while a positive correlation is observed between disulphide, disulphide/native thiol and disulphide/total thiol levels.
CONCLUSION
This study is first in the literature which found that the patients with celiac disease the dynamic thiol/disulphide balance shifts through disulphide form compared to the control group.
Core tip: To the best of our knowledge, for the first time in this study, total and native thiol levels in celiac patients were found lower compared to the control group while disulphide level, disulphide/total thiol and disulphide/native thiol ratios were found to be higher. Also, this study is first in which a negative correlation between celiac autoantibodies and native thiol, and total thiol levels and native thiol/total thiol ratio is observed while there is a positive correlation between disulphide level and disulphide/native thiol and disulphide/total thiol ratios.
