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©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
Comparison of the conventional tube and erythrocyte-magnetized technology in titration of red blood cell alloantibodies
Xue-Hua He, Hong Yan, Chun-Yan Wang, Xue-Yun Duan, Jia-Jia Qiao, Xiao-Jun Guo, Hong-Bin Zhao, Dong Ren, Jian-She Li, Qiang Zhang
Xue-Hua He, Jia-Jia Qiao, Xiao-Jun Guo, Hong-Bin Zhao, Dong Ren, Department of Blood Transfusion, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan 030032, Shanxi Province, China
Hong Yan, Jian-She Li, Department of Blood Transfusion, The Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
Chun-Yan Wang, Department of Blood Transfusion, Shanxi Cancer Hospital, Taiyuan 030013, Shanxi Province, China
Xue-Yun Duan, Department of Blood Transfusion, Shanxi Cardiovascular Hospital, Taiyuan 030024, Shanxi Province, China
Qiang Zhang, Department of Clinical Laboratory, Taiyuan Blood Center, Institute of Blood Transfusion Technology, Taiyuan 030024, Shanxi Province, China
Author contributions: He XH and Yan H contributed equally; He XH, Qiao JJ, and Guo XJ designed the study; Zhao HB, and Ren D wrote the manuscript; Yan H, and Li JS reviewed and edited; Wang CY projected administration; Duan XY performed the experiments; Zhang Q analysed the data; All authors have read and agreed to the published version of the manuscript.
Supported by Project of Shanxi Provincial Health Commission, No. 2021144.
Institutional review board statement: The study was approved by Ethics Committee of the Third Hospital of Shanxi Medical University, No. 2018-003.
Institutional animal care and use committee statement: This study was not involved any animal experiments.
Informed consent statement: All study participants or their legal guardian provided informed written consent about personal and medical data collection prior to study enrolment.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
https://creativecommons.org/Licenses/by-nc/4.0/ Corresponding author: Qiang Zhang, BSc, Chief Physician, Department of Clinical Laboratory, Taiyuan Blood Center, Institute of Blood Transfusion Technology, No. 185 Yingze West Street, Taiyuan 030024, Shanxi Province, China. qi59794383@163.com
Received: March 14, 2023
Peer-review started: March 14, 2023
First decision: April 7, 2023
Revised: April 16, 2023
Accepted: May 15, 2023
Article in press: May 15, 2023
Published online: May 27, 2023
ARTICLE HIGHLIGHTS
Research background
Magnetic red cell immunoseparation is a biomedical technique for separating and detecting small numbers of targeted cells. It has the advantages of high sensitivity, high precision, and easy operation and has been widely applied in the field of in vitro diagnostics and therapy.
Research motivation
Conventional separation methods can be time-consuming and involve complicated procedures, while magnetic red cell immunoseparation has the advantages of ease of use, high sensitivity, and precision. Therefore, this technology has been widely applied in in vitro diagnostics and therapy, attracting much attention from researchers and medical professionals.
Research objectives
This study aims to explore the application value of conventional test tubes and erythrocyte-magnetized technology (EMT) in red blood cell alloantibody titration to improve the safety of clinical blood transfusion.
Research methods
Parallel detection of antibody titers for different red blood cell blood groups using in vitro polyene test, tube antiglobulin test (AGT) and EMT screening for irregular antibodies.
Research results
The irregular antibody method for EMT screening could detect all immunoglobulin G and immunoglobulin M irregular antibodies, and the operation time is shorter than manual tube AGT. Furthermore, the EMT screening irregular antibody test was performed to detect its activity on O-type R (D) red blood cells, and the results reflected that it was normal. In addition, compared to the conventional tube method, the EMT screening method for irregular antibodies had lower costs and significantly higher detection efficiency.
Research conclusions
Compared with traditional in vitro methods, EMT screening for irregular antibodies has lower costs and significantly higher detection efficiency.
Research perspectives
The broad application prospects of red blood cell magnetization technology in medicine are evident. Technological advancements will further expand the application scope of this technology. For example, red blood cell magnetization technology can diagnose diseases like early cancer and cardiovascular diseases. In addition, this technology can be used for in vivo and in vitro research on the movement, interaction, and molecular processes of cells and pathogenic microorganisms.
