Published online May 27, 2023. doi: 10.4331/wjbc.v14.i3.62
Peer-review started: March 14, 2023
First decision: April 7, 2023
Revised: April 16, 2023
Accepted: May 15, 2023
Article in press: May 15, 2023
Published online: May 27, 2023
Magnetic red cell immunoseparation is a biomedical technique for separating and detecting small numbers of targeted cells. It has the advantages of high sensitivity, high precision, and easy operation and has been widely applied in the field of in vitro diagnostics and therapy.
Conventional separation methods can be time-consuming and involve complicated procedures, while magnetic red cell immunoseparation has the advantages of ease of use, high sensitivity, and precision. Therefore, this technology has been widely applied in in vitro diagnostics and therapy, attracting much attention from researchers and medical professionals.
This study aims to explore the application value of conventional test tubes and erythrocyte-magnetized technology (EMT) in red blood cell alloantibody titration to improve the safety of clinical blood transfusion.
Parallel detection of antibody titers for different red blood cell blood groups using in vitro polyene test, tube antiglobulin test (AGT) and EMT screening for irregular antibodies.
The irregular antibody method for EMT screening could detect all immunoglobulin G and immunoglobulin M irregular antibodies, and the operation time is shorter than manual tube AGT. Furthermore, the EMT screening irregular antibody test was performed to detect its activity on O-type R (D) red blood cells, and the results reflected that it was normal. In addition, compared to the conventional tube method, the EMT screening method for irregular antibodies had lower costs and significantly higher detection efficiency.
Compared with traditional in vitro methods, EMT screening for irregular antibodies has lower costs and significantly higher detection efficiency.
The broad application prospects of red blood cell magnetization technology in medicine are evident. Technological advancements will further expand the application scope of this technology. For example, red blood cell magnetization technology can diagnose diseases like early cancer and cardiovascular diseases. In addition, this technology can be used for in vivo and in vitro research on the movement, interaction, and molecular processes of cells and pathogenic microorganisms.