Application of urinary N-acetyl-β-D-glucosaminidase combined with serum retinol-binding protein in early detection of diabetic nephropathy

Table 1 Comparison of data between two groups

Indicators	Group A (n = 50)	Group B (n = 50)	Statistical value	P value
Gender, n (%)			χ2 = 0.164	0.685
Male	30 (60.00)	28 (56.00)
Female	20 (40.00)	22 (44.00)
Age (mean ± SD, yr)	43.12 ± 5.02	43.25 ± 5.12	t = 0.128	0.898
Duration of diabetes (mean ± SD, yr)	2.1 5 ± 0.52	2.23 ± 0.55	t = 0.747	0.457
Combined hyperlipidemia, n (%)			χ2 = 0.164	0.685
Yes	20 (40.00)	22 (44.00)
No	30 (60.00)	28 (56.00)
Combined hypertension, n (%)			χ2 = 0.170	0.680
Yes	18 (36.00)	20 (40.00)
No	32 (64.00)	30 (60.00)
Urine NAG (mean ± SD, U/L)	14.05 ± 2.20	19.45 ± 3.68	t = 8.906	< 0.001
Serum RBP (mean ± SD, mg/L)	43.56 ± 5.50	84.98 ± 15.70	t = 17.606	< 0.001

NAG: N-acetyl-β-D-glucosaminidase; RBP: Retinol-binding protein.

Table 2 Logistic regression analysis of the relationship between urinary N-acetyl-β-D-glucosaminidase, serum retinol-binding protein and diabetic nephropathy

Variable	B	SE	Wals	P value	OR	95%CI
						Upper limit	Lower limit
Constant	-9.366	1.808	26.839	0.000	0.000	-	-
Urine NAG (U/L)	0.568	0.111	26.338	0.000	1.765	1.421	2.192
Serum RBP (mg/L)	0.346	0.109	9.996	0.002	1.413	1.141	1.751

NAG: N-acetyl-β-D-glucosaminidase; RBP: Retinol-binding protein.

Table 3 Efficacy analysis of urinary N-acetyl-β-D-glucosaminidase and serum retinol-binding protein expression for predicting diabetic nephropathy

Indicators	AUC	95%CI of AUC	SE	P value	Cut-off value	Specificity	Sensitivity	Youden index
Urine NAG	0.867	0.796-0.939	0.036	0.000	11.855 (U/L)	0.980	0.860	0.840
Serum RBP	0.951	0.902-1.000	0.025	0.000	39.620 (mg/L)	0.980	0.780	0.640
Combined diagnosis	0.974	0.936-1.000	0.020	0.000	-	0.980	0.940

NAG: N-acetyl-β-D-glucosaminidase; RBP: Retinol-binding protein; AUC: Area under the curve.