Published online Dec 15, 2023. doi: 10.4239/wjd.v14.i12.1766
Peer-review started: October 7, 2023
First decision: October 17, 2023
Revised: October 24, 2023
Accepted: November 17, 2023
Article in press: November 17, 2023
Published online: December 15, 2023
Processing time: 68 Days and 7.6 Hours
Previous studies revealed that there are discriminating microbiota compositions between the Han and Dai populations. However, the underlying differences in the gut microbiota between the Han and Dai populations have not yet been elucidated.
We compared the differences in the gut microbiota in subjects with and without type 2 diabetes mellitus (T2DM) in the Han and Dai populations to explore the pathogenic mechanism of T2DM.
To identify the differences in the gut microbiota related to the occurrence of T2DM in the Han and Dai populations.
A total of 35 subjects of the Han population (15 healthy children, 8 adult healthy controls, and 12 adult T2DM patients) and 32 subjects of the Dai population (10 healthy children, 10 adult healthy controls, and 12 adult T2DM patients) were enrolled in this study. Fasting venous blood samples were collected from all the subjects for biochemical analysis. Fecal samples were collected from all the subjects for DNA extraction and 16S rRNA sequencing, which was followed by analyses of the gut microbiota composition.
Fasting plasma glucose levels and hemoglobin A1c were significantly increased in the T2DM patients. The gut microbiota of the Han population was significantly different from the Dai population in healthy children, healthy adults, and T2DM patients. Significant increases in Bacteroidetes were observed in T2DM patients from the Han population, while significant increases in Proteobacteria were observed in T2DM patients in the Dai population.
We observed significant differences in the gut microbiota in the Han and Dai populations, and these differences were influenced to a greater degree by age than by T2DM.
Our findings may provide additional insight for further study of the gut microbiota dysbiosis-related diseases in the Han and Dai populations. Future research should include a larger scale of samples and an investigation of the metabolic profile in order to confirm the relationship between an imbalance of the metabolism and gut microbiota alterations.