Tang LT, Feng L, Cao HY, Shi R, Luo BB, Zhang YB, Liu YM, Zhang J, Li SY. Comparative study of type 2 diabetes mellitus-associated gut microbiota between the Dai and Han populations. World J Diabetes 2023; 14(12): 1766-1783 [PMID: 38222790 DOI: 10.4239/wjd.v14.i12.1766]
Corresponding Author of This Article
Lei Feng, PhD, Chief Doctor, Full Professor, Department of Clinical Laboratory, Yan’an Hospital Affiliated to Kunming Medical University, No. 245 Renmin East Road, Kunming 650051, Yunnan Province, China. fngj2004@163.com
Research Domain of This Article
Microbiology
Article-Type of This Article
Case Control Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Diabetes. Dec 15, 2023; 14(12): 1766-1783 Published online Dec 15, 2023. doi: 10.4239/wjd.v14.i12.1766
Comparative study of type 2 diabetes mellitus-associated gut microbiota between the Dai and Han populations
Ling-Tong Tang, Lei Feng, Hui-Ying Cao, Rui Shi, Bei-Bei Luo, Yan-Bi Zhang, Yan-Mei Liu, Jian Zhang, Shuang-Yue Li
Ling-Tong Tang, Lei Feng, Hui-Ying Cao, Yan-Mei Liu, Jian Zhang, Shuang-Yue Li, Department of Clinical Laboratory, Yan’an Hospital Affiliated to Kunming Medical University, Kunming 650051, Yunnan Province, China
Rui Shi, Bei-Bei Luo, Yan-Bi Zhang, Department of Clinical Laboratory, Sixth Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan Province, China
Author contributions: Tang LT and Feng L contributed equally to this work; Tang LT and Feng L drafted the manuscript; Shi R contributed to recruiting patients; Tang LT, Cao HY, and Li SY collected the data; Zhang YB and Zhang J analyzed and interpreted the data; Liu YM contributed to conception and design; Luo BB contributed to administrative support; and all authors read and approved the final manuscript.
Supported bythe National Natural Science Foundation of China, No. 82160402; Special Fund for Training Leading Medical Talents in Yunnan Province, China, No. L-2019022.
Institutional review board statement: This study was reviewed and approved by the Ethics Committee of the Sixth Affiliated Hospital of Kunming Medical University (2023-kmykdx6f-66).
Informed consent statement: All study participants, or their legal guardians, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: No additional data are available.
STROBE statement: The authors have read the STROBE Statement-checklist of items, and the manuscript was prepared and revised according to the STROBE Statement-checklist of items.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Lei Feng, PhD, Chief Doctor, Full Professor, Department of Clinical Laboratory, Yan’an Hospital Affiliated to Kunming Medical University, No. 245 Renmin East Road, Kunming 650051, Yunnan Province, China. fngj2004@163.com
Received: October 7, 2023 Peer-review started: October 7, 2023 First decision: October 17, 2023 Revised: October 24, 2023 Accepted: November 17, 2023 Article in press: November 17, 2023 Published online: December 15, 2023 Processing time: 68 Days and 7.6 Hours
Abstract
BACKGROUND
The global prevalence of type 2 diabetes mellitus (T2DM) is increasing. T2DM is associated with alterations of the gut microbiota, which can be affected by age, illness, and genetics. Previous studies revealed that there are discriminating microbiota compositions between the Dai and the Han populations. However, the specific gut microbiota differences between the two populations have not been elucidated.
AIM
To compare the gut microbiota differences in subjects with and without T2DM in the Dai and Han populations.
METHODS
A total of 35 subjects of the Han population (including 15 healthy children, 8 adult healthy controls, and 12 adult T2DM patients) and 32 subjects of the Dai population (including 10 healthy children, 10 adult healthy controls, and 12 adult T2DM patients) were enrolled in this study. Fasting venous blood samples were collected from all the subjects for biochemical analysis. Fecal samples were collected from all the subjects for DNA extraction and 16S rRNA sequencing, which was followed by analyses of the gut microbiota composition.
RESULTS
No significant difference in alpha diversity was observed between healthy children and adults. The diversity of gut microbiota was decreased in T2DM patients compared to the healthy adults in both the Dai and Han populations. There was a significant difference in gut microbiota between healthy children and healthy adults in the Han population with an increased abundance of Bacteroidetes and decreased Firmicutes in children. However, this difference was less in the Dai population. Significant increases in Bacteroidetes in the Han population and Proteobacteria in the Dai population and decreases in Firmicutes in both the Han and Dai population were observed in T2DM patients compared to healthy adults. Linear discriminant analysis Effect Size analysis also showed that the gut microbiota was different between the Han and Dai populations in heathy children, adults, and T2DM patients. Four bacteria were consistently increased and two consistently decreased in the Han population compared to the Dai population.
CONCLUSION
Differences in gut microbiota were found between the Han and Dai populations. A significant increase in Bacteroidetes was related to the occurrence of T2DM in the Han population, while a significant increase in Proteobacteria was related to the occurrence of T2DM in the Dai population.
Core Tip: This study revealed that gut microbiota in the Han population is significantly different from the Dai population in healthy children, healthy adults, and patients with type 2 diabetes mellitus (T2DM). There was a significant difference in gut microbiota between healthy children and healthy adults in the Han population, but the difference was less in the Dai population. A significant increase in Bacteroidetes was observed in T2DM patients in the Han population, while a significant increase in Proteobacteria was observed in T2DM patients in the Dai population when compared to healthy controls.
