Attia MS, Ayman F, Attia MS, Yahya G, Zahra MH, Khalil MMI, Diab AAA. Mitigating diabetes-related complications: Empowering metformin with cholecalciferol and taurine supplementation in type 2 diabetic rats. World J Diabetes 2024; 15(8): 1778-1792 [PMID: 39192867 DOI: 10.4239/wjd.v15.i8.1778]
Corresponding Author of This Article
Fadwa Ayman, BSc, Master's Student, Research Assistant, Teaching Assistant, Department of Zoology, Faculty of Science, Faculty of Science Primary Building, Shaibet an Nakareyah, Zagazig 44519, Egypt. fadwaayman@zu.edu.eg
Research Domain of This Article
Biochemistry & Molecular Biology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Diabetes. Aug 15, 2024; 15(8): 1778-1792 Published online Aug 15, 2024. doi: 10.4239/wjd.v15.i8.1778
Mitigating diabetes-related complications: Empowering metformin with cholecalciferol and taurine supplementation in type 2 diabetic rats
Mai S Attia, Fadwa Ayman, Mohamed S Attia, Galal Yahya, Mansour H Zahra, Magdi Mohamed Ibrahim Khalil, Abdel Aziz A Diab
Mai S Attia, Fadwa Ayman, Mansour H Zahra, Abdel Aziz A Diab, Department of Zoology, Faculty of Science, Zagazig 44519, Egypt
Mohamed S Attia, Department of Pharmaceutics, Faculty of Pharmacy, Zagazig 44519, Egypt
Galal Yahya, Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt
Magdi Mohamed Ibrahim Khalil, Department of Biochemistry, Faculty of Medicine, Zagazig 44519, Egypt
Co-first authors: Mai S Attia and Fadwa Ayman.
Author contributions: Attia MaS and Ayman F contributed equally to this experimental study as they are co-first authors of this manuscript. Attia MaS, Ayman F and Diab AA designed the study; Attia MaS and Ayman F conducted the research experiments, Attia MaS and FA performed data collection and statistical analysis; Attia MoS, Ayman F, Attia MaS wrote the original draft; Attia MoS, Ayman F and Yahya G rewrote and revised the manuscript after editing; Diab AA, Zahra MH, Yahya G and Khalil MMI provided supervision; all authors have read and agreed to the published version of the manuscript.
Institutional animal care and use committee statement: The study was reviewed and approved by Institutional Review Board, No. ZU-IACUC/2/F/47/2022.
Conflict-of-interest statement: The authors declare that they have no competing interests.
Data sharing statement: The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Fadwa Ayman, BSc, Master's Student, Research Assistant, Teaching Assistant, Department of Zoology, Faculty of Science, Faculty of Science Primary Building, Shaibet an Nakareyah, Zagazig 44519, Egypt. fadwaayman@zu.edu.eg
Received: April 6, 2024 Revised: June 30, 2024 Accepted: July 17, 2024 Published online: August 15, 2024 Processing time: 111 Days and 1.7 Hours
Abstract
BACKGROUND
Type 2 diabetes is one of the most prevalent chronic diseases worldwide, significantly impacting patients' quality of life. Current treatment options like metformin (MET) effectively counteract hyperglycemia but fail to alleviate diabetes-associated complications such as retinopathy, neuropathy, nephropathy, hepatopathy, and cardiovascular diseases.
AIM
To propose the supplementation of cholecalciferol (CHO) and taurine (TAU) to enhance MET efficacy in controlling diabetes while minimizing the risk of associated complications.
METHODS
The study involved sixty rats, including ten non-diabetic control rats and fifty experimental rats with type 2 diabetes induced by streptozotocin. The experimental rats were further subdivided into positive control and treatment subgroups. The four treatment groups were randomly allocated to a single MET treatment or MET combined with supplements either CHO, TAU, or both.
RESULTS
Diabetic rats exhibited elevated levels of glucose, insulin, Homeostasis Model Assessment of Insulin Resistance (HOMA-IR), glycated hemoglobin percentage, lipid markers, aspartate aminotransferase, and malondialdehyde, along with reduced levels of antioxidant enzymes (catalase and superoxide dismutase). The administration of CHO and TAU supplements alongside MET in diabetic rats led to a noticeable recovery of islet mass. The antioxidative, anti-inflammatory, and anti-apoptotic properties of the proposed combination therapy significantly ameliorated the aforementioned abnormalities.
CONCLUSION
The supplementation of CHO and TAU with MET showed the potential to significantly improve metabolic parameters and protect against diabetic complications through its antioxidative, anti-inflammatory, and anti-apoptotic effects.
Core Tip: Supplementation of cholecalciferol and taurine in conjunction with metformin shows promise in enhancing the management of type 2 diabetes by addressing hyperglycemia and minimizing the risk of associated complications. This combination therapy demonstrates antioxidative, anti-inflammatory, and anti-apoptotic properties, leading to improvements in glucose and insulin levels, Homeostasis Model Assessment of Insulin Resistance, glycated hemoglobin%, lipid profiles, liver function markers, and oxidative stress markers in diabetic rats. The proposed regimen also promotes recovery of pancreatic islet mass, highlighting its potential to mitigate diabetic complications.
