Immunohistochemical and molecular genetic analyses of multiple sporadic gastrointestinal stromal tumors

Table 1 Details of the twelve gastrointestinal stromal tumors

Tumor number	Localization, distance from treitz ligament (cm)a	Tumor size (mm)	Resection	Mitotic counts	Risk stratification
#1	20	5 × 5	Wedge resection	< 5/50 HPF	Very low risk
#2	50	3 × 3	Wedge resection	< 5/50 HPF	Very low risk
#3	100	10 × 10	Wedge resection	< 5/50 HPF	Very low risk
#4	150	2 × 2	Wedge resection	< 5/50 HPF	Very low risk
#5	170	50 × 40	Partial resection	< 5/50 HPF	Intermediate risk
#6	180	10 × 10	Partial resection	< 5/50 HPF	Very low risk
#7	200	4 × 4	Partial resection	< 5/50 HPF	Very low risk
#8	220	5 × 5	Partial resection	< 5/50 HPF	Very low risk
#9	240	30 × 20	Partial resection	< 5/50 HPF	Low risk
#10	290	70 × 60	Partial resection	< 5/50 HPF	Intermediate risk
#11	295	5 × 5	Partial resection	< 5/50 HPF	Very low risk
#12	300	6 × 6	Partial resection	< 5/50 HPF	Very low risk

^aThe full length of the small intestine was 410 cm, HPF: High-power field. The tumor number #5 to #12 are resected together.

Table 2 Result of the deoxyribonucleic acid sequence

Sample	c-kit				PDGFRA
	Exon 9	Exon 11	Exon 13	Exon 17	Exon 12	Exon 14	Exon 18
Whole blood	WT	WT	WT	WT	silent	WT	WT
					mutation
Normal mucosa	WT	WT	WT	WT	silent	WT	WT
					mutation
Tumor #5	WT	WT	missense	WT	silent	WT	WT
			mutation		mutation
Tumor #10	WT	WT	missense	WT	silent	WT	WT
			mutation		mutation

PDGFRA: Platelet derived growth factor receptor A; WT: Wild-type.

Table 3 Discrimination of the multiple gastrointestinal stromal tumor in adult[6-10,12]

	Clinical features	Mutation	Hyperplasia of ICC	Family history	Muscularis propria involvement
Sporadic GIST	(-)	(+) Somatic mutation	(-)	(-)	(+)
Familial GIST	(+) Skin pigmentation	(+) Germline mutation	(+) Diffuse	(+)	(+)
Neurofibromatosis type 1 associated GIST	(+) Skin nodule and pigmentation	(-) rare	(+) Focal or mild	(+)	(+)
Metastatic disease	a	a	a	a	(-)

^aSame or similar to primary tumor. ICC: Interstitial cells of Cajal; GIST: Gastrointestinal stromal tumor.