N-myc downstream regulated gene 1 inhibition of tumor progression in Caco2 cells

Table 1 Primers used for the establishment of cell lines

Gene	Forward primer	Reverse primer
sgRNA1	5’-ATCCTCACCTACCATGACAT-3’	5’-ATGTCATGGTAGGTGAGGAT-3’
sgRNA2	5’-ACGCTGTGTGGGACTCCCAA-3’	5’-TTGGGAGTCCCACACAGCGT-3’
sgRNA3	5’-GTTCATGCCGATGTCATGG-3’	5’-ACCATGACATCGGCATGAAC-3’
NDRG1	5’-TTTGGTGCATTTAACAGCGCAGTCT-3’	5’-CAGGAAGTCCCAGGCAAAAAGAAAC-3’

NDRG1: N-myc downstream regulated gene 1; sgRNA: Single-guide RNA.

Table 2 Primers used for quantitative polymerase chain reaction

Gene	Forward primer	Reverse primer
GAPDH	5’-GCACCGTCAAGGCTGAGAA-3’	5’-TGGTGAAGACGCCAGTGGA-3’
NDRG1	5’-ATGTCTCGGGAGATGCAGGATGTAG-3’	5’-CTAGCAGGAGACCTCCATGGACTTG-3’

NDRG1: N-myc downstream regulated gene 1.