Basic Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Dec 15, 2022; 14(12): 2313-2328
Published online Dec 15, 2022. doi: 10.4251/wjgo.v14.i12.2313
N-myc downstream regulated gene 1 inhibition of tumor progression in Caco2 cells
Yi-Xiao He, Hong Shen, Yu-Zhu Ji, Hai-Rong Hua, Yu Zhu, Xiang-Fei Zeng, Fang Wang, Kai-Xin Wang
Yi-Xiao He, Yu-Zhu Ji, Department of Pathology, Mianyang Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Mianyang 621000, Sichuan Province, China
Yi-Xiao He, Hai-Rong Hua, Fang Wang, Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, Kunming 650500, Yunnan Province, China
Hong Shen, Department of Pathology, Zhaotong First People’s Hospital, Zhaotong 657000, Yunnan Province, China
Yu Zhu, School of Nursing, Henan Vocational College of Applied Technology, Kaifeng 450000, Henan Province, China
Xiang-Fei Zeng, Department of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610000, Sichuan Province, China
Kai-Xin Wang, Department of Pathology, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen 518052, Guangdong Province, China
Author contributions: He YX, Shen H and Ji YZ have contributed equally to the work; He YX, Shen H and Ji YZ performed the experiments, analyzed the data and wrote the manuscript; Hua HR and Zhu Y contributed analysis tools, acquired and analyzed data; Zeng XF helped perform the analysis with constructive discussions; Wang F and Wang KX conceived and designed the experiments as well as an acquired research grant; and all authors read and approved the final version of the article.
Supported by the National Natural Science Foundation of China, No. 81260361; and Incubation Project of Mianyang Central Hospital, No. 2020FH05.
Conflict-of-interest statement: He YX, Shen H, Ji YZ, Zhu Y, Zeng XF and Wang KX were previously postgraduate students at Kunming Medical University. Wang Fang was a teacher at Kunming Medical University. All the authors report no relevant conflicts of interest for this article.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Kai-Xin Wang, MD, Associate Chief Physician, Department of Pathology, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), No. 89 Taoyuan Road, Nanshan District, Shenzhen 518052, Guangdong Province, China.
Received: July 14, 2022
Peer-review started: July 14, 2022
First decision: September 26, 2022
Revised: October 17, 2022
Accepted: November 22, 2022
Article in press: November 22, 2022
Published online: December 15, 2022
Research background

In American colorectal cancer patients, the expression of N-myc downstream regulated gene 1 (NDRG1) in primary colorectal cancer was always less than that in adjacent normal tissues. Our study on clinical samples showed the opposite. Therefore, the effect of NDRG1 in cancer may be related to the ethnic backgrounds of colorectal cancer. The future research direction is to find out whether the role of NDRG1 in the development of colorectal tumors is related to ethnic differences through in vivo and in vitro experiments.

Research motivation

This study identifies the in vitro role of NDRG1 in Caucasian large intestine tumors as inhibition of tumor cell invasion and migration.

Research objectives

This study only showed the in vitro effect of NDRG1 in Caucasian large intestine tumors, and the question remains to be solved about the effect of NDRG1 in large intestine tumors of other races.

Research methods

RNA extraction and quantitative polymerase chain reaction, western blotting, cell counting kit-8 assay, assessment of cell cycle by flow cytometric analysis, assessment of apoptosis by flow cytometric analysis, 24-transwell for invasion and migration were used in the experiments. GraphPad Prism 7.0. student’s t-test was used for statistical analysis.

Research results

The primary objective was to investigate the role of NDRG1 in the development and progression of colorectal tumors. The objective has been to investigate the in vitro role of NDRG1 in the development of Caucasian human bowel tumors. The significance of achieving these goals for future research in this area is to provide clinicians with a theoretical basis for selecting therapies for colorectal cancer.

Research conclusions

The research topic is NDRG1 and the key problem to be solved is the role of NDRG1 in the occurrence and development of colorectal tumors. The results can provide a certain basis for the selection of treatment options for colorectal tumors.

Research perspectives

In 2004, our research group published an article in the World Journal of Gastroenterology on our research data with a large clinical sample (150 cases): “Correlation of N-myc downstream-regulated gene 1 over-expression with progressive growth of colorectal neoplasm.”, which carried significance on the association of NDRG1 with colorectal cancer progression.