Potential microRNA panel for the diagnosis and prediction of overall survival of hepatocellular carcinoma with hepatitis B virus infection

Table 1 Clinical features of the hepatitis B virus cirrhosis and normal controls, n (%)

Variables	HBV cirrhosis (n = 40)	Normal controls (n = 40)	P value
Average age (yr)	42.38 ± 8.93	44.58 ± 11.79	0.3561
Sex			0.1332
Male	26 (65.0)	32 (80.0)
Female	14 (35.0)	8 (20.0)
Alcohol consumption			0.0562
Ever or current	31 (77.5)	23 (57.5)
Never	9 (22.5)	17 (42.5)

¹Student-t test.

²Two-sided χ² test. HBV: Hepatitis B virus.

Table 2 The relative concentration of miRNAs in hepatitis B virus cirrhosis samples and control samples¹ (n = 40)

miRNA	HBV cirrhosis	Normal control	Fold change2	P value
miR-224	14.85	6.67	2.23	< 0.0001
miR-122	38.87	81.44	0.48	< 0.0001
miR-375	24.75	52.53	0.47	< 0.0001
miR-143	7.32	15.10	0.48	< 0.0001

¹miRNA concentrations are presented as the mean (SE).

²Hepatitis B virus cirrhosis/normal control. HBV: Hepatitis B virus.

Table 3 Risk score analysis of hepatitis B virus cirrhosis patients and normal controls

Score	0-2.016	> 20.16	PPV	NPV
Normal subject (n = 40)	34	6		0.92
HBV cirrhosis (n = 40)	3	37	0.86
Total	37	43

PPV: Positive predictive value; NPV: Negative predictive value; HBV: Hepatitis B virus.

Table 4 The overall survival of patients with different expression level (mo)

	miR-224	miR-122	miR-375	miR-143
Higher expression	34.45 ± 13.09	42.19 ± 12.98	40.75 ± 14.12	39.38 ± 15.08
Lower expression	41.72 ± 13.42	34.75 ± 13.39	34.70 ± 12.60	35.89 ± 11.77