Basic Study
Copyright ©The Author(s) 2019.
World J Gastrointest Oncol. Sep 15, 2019; 11(9): 705-716
Published online Sep 15, 2019. doi: 10.4251/wjgo.v11.i9.705
Table 1 The forward and reverse primers of qRT-PCR
GenePrimers sequence (5-3’)
MiR-331F: GCCCCTGGGCCTATCCTAGAA
R: ACGCGTCGACTTTTAGGGCTAAGTTGCTTC
MSI1F: CTCCAAAACAATTGACCCTA
R: GCTCAAAATATTGCTTCACG
U6F: AGAGCCTGTGGTGTCCG
R: CATCTTCAAAGCACTTCCC
GAPDHF: ATGGGGAAGGTGAAG GTCG
R: GGGGTCATTGATGGCAACAAT A
Table 2 Relationship between miR-331 expression and clinic-pathological characteristics of gastric cancer patients
CharacteristicsCasesmiR-331
P value
HighLow
Age (yr)0.34
≥ 55411625
< 5537829
Gender0.16
Male431330
Female351124
Tumor size (mm)0.07
≤ 3251015
> 3531439
Lymph nodes metastasis0.03a
Yes21813
No571641
TNM stage0.02a
I-II551540
III-IV23914