MicroRNA-331 inhibits development of gastric cancer through targeting musashi1

Table 1 The forward and reverse primers of qRT-PCR

Gene	Primers sequence (5-3’)
MiR-331	F: GCCCCTGGGCCTATCCTAGAA
	R: ACGCGTCGACTTTTAGGGCTAAGTTGCTTC
MSI1	F: CTCCAAAACAATTGACCCTA
	R: GCTCAAAATATTGCTTCACG
U6	F: AGAGCCTGTGGTGTCCG
	R: CATCTTCAAAGCACTTCCC
GAPDH	F: ATGGGGAAGGTGAAG GTCG
	R: GGGGTCATTGATGGCAACAAT A

Table 2 Relationship between miR-331 expression and clinic-pathological characteristics of gastric cancer patients

Characteristics	Cases	miR-331		P value
		High	Low
Age (yr)				0.34
≥ 55	41	16	25
< 55	37	8	29
Gender				0.16
Male	43	13	30
Female	35	11	24
Tumor size (mm)				0.07
≤ 3	25	10	15
> 3	53	14	39
Lymph nodes metastasis				0.03a
Yes	21	8	13
No	57	16	41
TNM stage				0.02a
I-II	55	15	40
III-IV	23	9	14

^aP < 0.05 was considered significant. Statistical analyses were performed by the χ² test.