Three novel NEIL1 promoter polymorphisms in gastric cancer patients

Figure 1 Identification of novel NEIL1 promoter polymorphisms by PCR-SSCP and subsequent sequencing analyses. A: Schematic map of the NEIL1 promoter region. The three genetic polymorphisms identified in this study are mapped, and the 8 PCR-amplified regions (Re 1-Re 8) and restriction enzyme sites are shown; B: Identification of c.-3769C>T, c.-3170T>G, and c.-2681TA[8] polymorphisms in the NEIL1 promoter. The panels on the left show representative results of the PCR-SSCP analysis. The electropherograms on the right show the results of the sequencing analysis. Only the lowermost panels show the results of sequencing after subcloning the PCR product; the others show the results of direct sequencing.