Prognostic significance of exportin-5 in hepatocellular carcinoma

Figure 1 Exportin-5 in hepatocellular carcinoma diagnosis. A: Exportin-5 expression in normal vs tumor; B: Paired tissue comparison; C: Kaplan-Meier curve for overall survival; D: Progression-free survival; E: International Cancer Genome Consortium patients’ survival analysis by expression level; F: Gender-based expression differences. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.

Figure 2 Prognostic tools for hepatocellular carcinoma. A: Survival prediction nomograms; B: Receiver operating characteristic curves for 1, 3, 5-year predictions; C: Nomogram validation. XPO5: Exportin-5; AUC: Area under the curve; OS: Overall survival.

Figure 3 Exportin-5 expression in hepatocellular carcinoma. A-C: Box plots by clinical features; D: Heatmap of expression and clinicopathologic associations. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. XPO5: Exportin-5.

Figure 4 Exportin-5 expression in liver cell lines. A and B: Western blot detection in WRL68, SK-HEP-1, SNU-449, Li-7, and SNU-398; C: qPCR analysis of mRNA levels. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. XPO5: Exportin-5.

Figure 5 Lentivirus transfection knockdown of exportin-5. A-F: Validation of transfection efficiency for exportin-5 knockdown, showcasing the effectiveness of the approach; G: The experimental design incorporates a screening process for determining the optimal viral multiplicity of infection. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. XPO5: Exportin-5; MOI: Multiplicity of infection.

Figure 6 Knockdown of exportin-5 can inhibit cell invasion and migration in SNU-398 and SNU-449 cells. SNU-398 and SNU-449 cells were transfected with shRNA-NC or shRNA- exportin-5. A and E: Cell invasion of SNU-398 were detected by transwell assays; B and G: Cell invasion of SNU-449 were detected by transwell assays; C and F: Wound-healing assays of SNU-398 were performed to assess cell migration; D and H: Wound-healing assays of SNU-449 were performed to assess cell migration. Results were mean ± SD for three individual experiments. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. XPO5: Exportin-5; NC: Control group.