Sm-like 5 knockdown inhibits proliferation and promotes apoptosis of colon cancer cells by upregulating p53, CDKN1A and TNFRSF10B

Figure 1 The expression of Sm-like 5 in colon cancer and its relationship with prognosis. A: The expression level of Sm-like 5 (LSM5) in colon tumor tissue and paired normal tissue according to the Gene Expression Profiling Interactive Analysis website; B: Immunohistochemistry analysis of LSM5 according to the Human Protein Atlas database; C: The prognostic analysis of LSM5 expression in patients with colon cancer. COAD: Chronic obstructive air way disease.

Figure 2 The expression level of Sm-like 5 decreased after knockdown. A: High expression of Sm-like 5 (LSM5) in 4 colon cancer cell lines confirmed by real-time quantitative polymerase chain reaction (RT-qPCR); B-E: LSM5 expression decreased significantly after knockdown, as confirmed by RT-qPCR (B and C) and Western blotting (D and E). LSM5: Sm-like 5.

Figure 3 Knockdown of Sm-like 5 inhibited the proliferation of colon cancer cells. A and B: Cell counting assay; C and D: MTT assay; E and F: Colony-forming assay. LSM5: Sm-like 5.

Figure 4 Knockdown of Sm-like 5 improved the prognosis of colon cancer cells. A and B: Flow cytometry. LSM5: Sm-like 5.

Figure 5 Microarray assay and differentially expressed genes analysis after Sm-like 5 knockdown. Scatter plot, volcano plot and hierarchical clustering analysis showing 757 upregulated genes and 1318 downregulated genes following Sm-like 5 knockdown. A: Scatter plot; B: Volcano plot; C: Hierarchical clustering analysis. DEG: differentially expressed gene; LSM5: Sm-like 5.

Figure 6 Ingenuity pathway analysis of differentially expressed gene after Sm-like 5 knockdown. A: Classical pathway analysis showing the 15 signalling pathways associated with the differentially expressed gene (DEGs); B: Disease and functional analysis showed the top 15 diseases and functions enriched by DEGs; C: Interaction network analysis showing the interactions between DEGs after Sm-like 5 knockdown.

Figure 7 Knockdown of Sm-like 5 promoted the expression of p53, cyclin-dependent kinase inhibitor 1A and tumor necrosis factor receptor superfamily 10B. A: Real-time quantitative polymerase chain reaction showed that the expression of several downstream molecules changed following Sm-like 5 (LSM5) knockdown; B and C: Western blotting showed that the expression of P53, cyclin-dependent kinase inhibitor 1A and MDM2 was upregulated after LSM5 knockdown. LSM5: Sm-like 5.