Genome-wide methylation profiling of early colorectal cancer using an Illumina Infinium Methylation EPIC BeadChip

Figure 1 The heatmap and volcano of the differentially methylated sites. A: Adenoma group; B: Delta β data. Ca: Cancer group.

Figure 2 The distribution of differentially methylated sites. A: Examples of the distribution of differentially methylated sites (DMSs) on chromosome 1, 2, and 3. The orange and blue dots represent the CpGs which are Δβ > 0 and Δβ < 0 respectively, the green peak represents the density of probes; B: Schematic map of the genomic DMSs, where red and blue represent the CpGs which are Δβ > 0 and Δβ < 0 respectively.

Figure 3 Distribution of differentially methylated sites. A: Distribution of differentially methylated sites (DMSs) in gene structural elements; B: Distribution of DMSs in and around CpG island.

Figure 4 Gene ontology analysis. A: Gene ontology (GO) function distribution of differentially methylated sites; B: Bubble diagram of GO enrichment results. GO: Gene ontology; BP: Biological process; CC: Cellular component; MF: Molecular function.

Figure 5 Kyoto Encyclopedia of Genes and Genomes analysis. A: Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway classification map; B: Bubble diagram of KEGG enrichment pathways. KEGG: Kyoto Encyclopedia of Genes and Genomes.