Proteomic signatures of infiltrative gastric cancer by proteomic and bioinformatic analysis

Figure 1 Schematic diagram illustrating the procedure of high performance liquid chromatography tandem mass spectrometry for proteomic analysis. HPLC-MS/MS: High performance liquid chromatography tandem mass spectrometry.

Figure 2 Volcano map of significant differentially expressed proteins. A and B: Representative images of hematoxylin and eosin stained infiltrative gastric cancer (IGC) (A) and normal gastric tissues (B); C: Base peak chromatogram of tissue sample on Orbitrap LTQ-LC/MS-CID activation; D: Expanded region of a single FTMS full scan with a mass range of m/z 350-1800, and ion peaks with double or higher charge (612.30, Z = 3); E: Volcano map showing differentially expressed proteins between IGC and normal tissues. P-adj: Corrected P value; Fold change: Ratio of expression levels in cancer and adjacent tissues.

Figure 3 Protein interaction networks of differentially expressed proteins. A and B: The interaction network of the significantly up-regulated proteins (A) in infiltrative gastric cancer (IGC) and the top 10 proteins (B); C: The interaction network of the significantly down-regulated proteins (C) in IGC and the top 10 proteins (D); E and F: Representative immunoblots showing the expression levels of specific up-regulated (E) and down-regulated (F) proteins in IGC tissues. ^aP < 0.05; ^cP < 0.001.

Figure 4 Gene Ontology of differentially expressed proteins. A and B: Histograms showing the significant Gene Ontology (GO) terms of up-regulated (A) and down-regulated (B) proteins; C and D: Topological network diagram of the GO terms of up-regulated (C) and down-regulated (D) proteins; E-G: Venn diagrams showing significantly enriched BP (E), CC (F) and MF (G) terms in the up-regulated proteins; H-J: Venn diagrams showing significantly enriched BP (H), CC (I) and MF (J) terms in the down-regulated proteins.

Figure 5 Enriched Kyoto Encyclopedia of Genes and Genomes pathways in differentially expressed proteins. A and B: Venn diagram of Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment in the up-regulated protein group (A) and significantly enriched KEGG pathways (B); C and D: Venn diagram of KEGG enrichment in the down-regulated protein group (C) and significantly enriched KEGG pathways (D).