Xin MJ, Yuan Y. Centromere protein A knockdown inhibits rectal cancer through O6-methylguanine DNA methyltransferase/protein tyrosine phosphatase nonreceptor type 4 axis. World J Gastrointest Oncol 2025; 17(4): 102619 [DOI: 10.4251/wjgo.v17.i4.102619]
Corresponding Author of This Article
Ming-Jie Xin, BM, Medical College, Henan Vocational University of Science and Technology, No. 6 East Section of Wenchang Avenue, Zhoukou 466000, Henan Province, China. xinmingjie_1985@126.com
Research Domain of This Article
Oncology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastrointest Oncol. Apr 15, 2025; 17(4): 102619 Published online Apr 15, 2025. doi: 10.4251/wjgo.v17.i4.102619
Centromere protein A knockdown inhibits rectal cancer through O6-methylguanine DNA methyltransferase/protein tyrosine phosphatase nonreceptor type 4 axis
Ming-Jie Xin, Yong Yuan
Ming-Jie Xin, Yong Yuan, Medical College, Henan Vocational University of Science and Technology, Zhoukou 466000, Henan Province, China
Author contributions: Xin MJ designed the research study and performed the research; Yuan Y analyzed the data and wrote the manuscript; All authors have read and approved the final manuscript.
Institutional review board statement: This study was reviewed and approved by the Ethic Committee of Medical College of Henan Vocational University of Science and Technology (Approval No. HVUYL414101416920231017001), and all participants signed a written informed consent.
Institutional animal care and use committee statement: All animal experiments were approved by the Animal Ethics Committee of the Medical College of Henan Vocational University of Science and Technology (Approval No. HVUYL414101416920240603001).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE Guidelines, and the manuscript was prepared and revised according to the ARRIVE Guidelines.
Data sharing statement: The datasets used during the present study are available from the corresponding author upon reasonable request (E-mail: xinmingjie_1985@126.com).
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Ming-Jie Xin, BM, Medical College, Henan Vocational University of Science and Technology, No. 6 East Section of Wenchang Avenue, Zhoukou 466000, Henan Province, China. xinmingjie_1985@126.com
Received: October 24, 2024 Revised: January 7, 2025 Accepted: February 25, 2025 Published online: April 15, 2025 Processing time: 153 Days and 7.8 Hours
Core Tip
Core Tip: This study suggested that centromere protein A (CENPA) was upregulated in rectal cancer tissues and cell lines. CENPA overexpression promoted proliferation, invasion and epithelial-mesenchymal transition, and inhibited apoptosis in rectal cancer cells. Whereas CENPA knockdown showed the opposite results. Additionally, CENPA knockdown inhibited xenograft tumor growth in vivo. Mechanistically, CENPA inhibited O6-methylguanine DNA methyltransferase (MGMT) expression by promoting DNA methyltransferase 1-mediated MGMT promoter methylation. MGMT interacted with protein tyrosine phosphatase nonreceptor type 4 (PTPN4) and increased PTPN4 protein stability. CENPA knockdown inhibited rectal cancer progression through regulating the MGMT/PTPN4 axis.
