Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Jun 15, 2024; 16(6): 2769-2780
Published online Jun 15, 2024. doi: 10.4251/wjgo.v16.i6.2769
N-glycosylation of Wnt3 regulates the progression of hepatocellular carcinoma by affecting Wnt/β-catenin signal pathway
Xin-Zhan Zhang, Xiao-Chuan Mo, Zhu-Ting Wang, Rong Sun, Da-Quan Sun
Xin-Zhan Zhang, Xiao-Chuan Mo, Zhu-Ting Wang, Rong Sun, Da-Quan Sun, Department of Biochemistry and Molecular Biology & Research Center for Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou Province, China
Author contributions: Zhang XZ, Sun DQ, Mo XC, Wang ZT, Sun R contributed to the conception of the study; Zhang XZ performed the experiment; Zhang XZ, Mo XC contributed significantly to analysis and manuscript preparation; Zhang XZ, Sun DQ performed the data analyses and wrote the manuscript; Zhang XZ, Sun DQ, Mo XC, Wang ZT, Sun R helped perform the analysis with constructive discussions.
Supported by National Natural Science Foundation of China, No. 81560390; the Guizhou Medical University Cultivation Project of the National Natural Science Foundation of China, No. 22NSFCP02; and Basic Research Project of Science and Technology Department of Guizhou Province, No. ZK[2024] General 136.
Institutional animal care and use committee statement: The study was reviewed and approved by the Animal Care Welfare Committee of Guizhou Medical University.
Conflict-of-interest statement: Dr. Sun has nothing to disclose.
Data sharing statement: No data available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Da-Quan Sun, PhD, Assistant Professor, Department of Biochemistry and Molecular Biology & Research Center for Basic Medical Sciences, Guizhou Medical University, Ankang Avenue, Machang Town, Guian New District, Guiyang 550025, Guizhou Province, China. sundq04@sina.com
Received: March 7, 2024
Revised: April 25, 2024
Accepted: April 28, 2024
Published online: June 15, 2024
Processing time: 99 Days and 16.8 Hours
Core Tip

Core Tip: Our study reveals that Wnt3 undergoes N-glycosylation modification at two specific sites (Asn90 and Asn301). Mutation of these sites impairs the stability and function of Wnt3, reducing its binding ability to FZD7 and downregulating the Wnt/β-catenin signaling pathway. Consequently, cell proliferation, migration, and invasion are attenuated in hepatocellular carcinoma cells. These findings suggest that targeting Wnt3 N-glycosylation could be a potential therapeutic strategy for liver cancer.