Fine-needle aspiration technique under endoscopic ultrasound guidance: A technical approach for RNA profiling of pancreatic neoplasms

doi:10.4251/wjgo.v16.i6.2663

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 16, Issue 6

This Article

Academic Content and Language Evaluation of This Article

CrossCheck and Google Search of This Article

Academic Rules and Norms of This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (269)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-2) series, Tables (1-1) series.

Item

Count

PDF

HTML

179

Figures (1-2)

Tables (1-1)

Sum=245

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

Download

Sum=20

Jun 15, 2024 (publication date) through Jun 29, 2024

Times Cited of This Article

Times Cited (0)

Journal Information of This Article

Publication Name

World Journal of Gastrointestinal Oncology

ISSN

1948-5204

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Gastrointest Oncol. Jun 15, 2024; 16(6): 2663-2672
Published online Jun 15, 2024. doi: 10.4251/wjgo.v16.i6.2663

Fine-needle aspiration technique under endoscopic ultrasound guidance: A technical approach for RNA profiling of pancreatic neoplasms

Sabina Sherafedinovna Seyfedinova, Olga Aleksandrovna Freylikhman, Polina Sergeevna Sokolnikova, Konstantin Aleksandrovich Samochernykh, Anna Aleksandrovna Kostareva, Olga Viktorovna Kalinina, Evgeniy Gennadievich Solonitsyn

Sabina Sherafedinovna Seyfedinova, Olga Aleksandrovna Freylikhman, Evgeniy Gennadievich Solonitsyn, Research Laboratory of Digestive Cancer, Almazov Medical Research Centre, Saint-Petersburg 197341, Russia

Polina Sergeevna Sokolnikova, Research Laboratory of Molecular and Cellular Modeling and Gene Therapy, Almazov Medical Research Centre, Saint-Petersburg 197341, Russia

Konstantin Aleksandrovich Samochernykh, Head of Russian Neurosurgical Institute Named after Prof. A. L. Polenova, Russian Neurosurgical Institute Named after Prof. A. L. Polenova, Saint-Petersburg 191014, Russia

Anna Aleksandrovna Kostareva, Head of Institute of Molecular Biology and Genetics, Almazov National Medical Research Centre, Saint-Petersburg 197341, Russia

Olga Viktorovna Kalinina, Research Laboratory of Autoimmune and Autoinflammatory Diseases, Almazov National Medical Research Centre, Saint-Petersburg 197341, Russia

Co-first authors: Sabina Sherafedinovna Seyfedinova and Olga Aleksandrovna Freylikhman.

Author contributions: Freylikhman OA as a specialist in molecular biology conducted technical part of the investigation and descripted methods and results in the article, contributed in the methodology development. Seyfedinova SS and Freylikhman OA are co-first authors of this manuscript. Seyfedinova SS, Freylikhman OA, and Sokolnikova PS contributed to the data curation and investigation; Seyfedinova SS, Freylikhman OA, Samochernykh KA, Kostareva AA, Kalinina OV, and Solonitsyn EG were involved in the methodology of this study; Seyfedinova SS and Freylikhman OA participated in the writing-original draft; Samochernykh KA and Kostareva AA contributed to the supervision of this manuscript; Kostareva AA, Kalinina OV, and Solonitsyn EG were involved in the validation and editing of this article; Kalinina OV and Solonitsyn EG contributed to the conceptualization.

Supported by the Ministry of Science and Higher Education of the Russian Federation, No. 075-15-2022-301.

Institutional review board statement: All samples were obtained for scientific research according to the Helsinki Declaration and approved by the Almazov National Medical Centre ethics committee (Approval No. 2101-23).

Informed consent statement: All study participants, or their legal guardians, provided informed written consent before study enrollment.

Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.

Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at seysabina000@gmail.com. No additional data are available.

Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/

Corresponding author: Sabina Sherafedinovna Seyfedinova, Doctor, Research Scientist, Research Laboratory of Digestive Cancer, Almazov Medical Research Centre, Akkuratova Av. 2, Saint-Petersburg 197341, Russia. seysabina000@gmail.com

Received: December 26, 2023
Revised: February 18, 2024
Accepted: April 7, 2024
Published online: June 15, 2024
Processing time: 171 Days and 19.2 Hours

Core Tip

Core Tip: In this study, the use of RNA from endoscopic ultrasound-guided, fine-needle aspiration (EUS-FNA) samples of cystic and solid pancreatic neoplasms, including samples of low-quality RNA, was demonstrated. The process of sample preparation from EUS-FNA material is detailed along with the necessary criteria for assessing RNA quality for effective molecular biological analysis. Issues regarding the dependence of RNA quality on the type of neoplasm and material are identified. Modification of the library preparation protocol made it possible to obtain libraries for samples with RNA integrity number equivalent values of three and above, meeting the quality criteria for purity and concentration.