Li Y, Yuan SL, Yin JY, Yang K, Zhou XG, Xie W, Wang Q. Differences of core genes in liver fibrosis and hepatocellular carcinoma: Evidence from integrated bioinformatics and immunohistochemical analysis. World J Gastrointest Oncol 2022; 14(7): 1265-1280 [PMID: 36051101 DOI: 10.4251/wjgo.v14.i7.1265]
Corresponding Author of This Article
Qi Wang, MD, PhD, Reader (Associate Professor), Center of Liver Diseases, Beijing Ditan Hospital, Capital Medical University, No. 8 East Jingshun Street, Chaoyang District, Beijing 100015, China. wangqidl04@ccmu.edu.cn
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Clinical and Translational Research
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastrointest Oncol. Jul 15, 2022; 14(7): 1265-1280 Published online Jul 15, 2022. doi: 10.4251/wjgo.v14.i7.1265
Differences of core genes in liver fibrosis and hepatocellular carcinoma: Evidence from integrated bioinformatics and immunohistochemical analysis
Yue Li, Shou-Li Yuan, Jing-Ya Yin, Kun Yang, Xin-Gang Zhou, Wen Xie, Qi Wang
Yue Li, Kun Yang, Xin-Gang Zhou, Department of Pathology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
Yue Li, Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China
Shou-Li Yuan, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Beijing 100101, China
Jing-Ya Yin, Wen Xie, Qi Wang, Center of Liver Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
Author contributions: Li Y and Yuan SL contributed equally to this work; Wang Q and Xie W designed the research and were co-corresponding authors; Yuan SL, Li Y, Yin JY, Yang K and Zhou XG performed the experiments; Yuan SL and Li Y analyzed the data; Yuan SL, Li Y and Wang Q wrote the manuscript; Yuan SL provided vital reagents and analytical tools; all authors read and approved the final manuscript.
Supported bythe Beijing Natural Science Foundation, No. 7222097; Beijing Hospitals Authority the Digestive Medical Coordinated Development Center, No. XXZ0401; National Natural Science Foundation of China, No. 82000555 and No. 81900547; and Beijing Municipal Science and Technology Commission, No. D171100003117005.
Institutional review board statement: The study protocol conforms to the ethical guidelines of the 1975 Declaration of Helsinki as reflected in a priori approval by the Ethics Committee of Beijing Ditan Hospital No. 2021-034-01.
Informed consent statement: Patients were not required to give informed consent to the study because the analysis used anonymous clinical data and liver tissue samples.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: Publicly available datasets were analyzed in this study, which can be found here: GSE14323, GSE36411 and GSE89377. Technical appendix, statistical code, and data set available from the corresponding author at wangqidl04@ccmu.edu.cn. No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Qi Wang, MD, PhD, Reader (Associate Professor), Center of Liver Diseases, Beijing Ditan Hospital, Capital Medical University, No. 8 East Jingshun Street, Chaoyang District, Beijing 100015, China. wangqidl04@ccmu.edu.cn
Received: January 25, 2022 Peer-review started: January 25, 2022 First decision: May 9, 2022 Revised: May 18, 2022 Accepted: June 26, 2022 Article in press: June 26, 2022 Published online: July 15, 2022 Processing time: 168 Days and 22.7 Hours
Core Tip
Core Tip: GSE14323, GSE36411, and GSE89377 are available from the Gene Expression Omnibus database. Forty-five differentially expressed genes and 10 hub genes were identified between cirrhotic and healthy livers. quantitative polymerase chain reaction, Western blot, and immunohistochemical analyses showed that decorin (DCN), dermatopontin (DPT), and SRY-box transcription factor 9 (SOX9) were highly expressed in the CCl4-induced cirrhotic mouse model. The expression level of SOX9 was also significantly increased in HCC patients,and was associated with the fibrosis stage.. However, overexpression of DPT was only observed in patients with liver fibrosis. The Kaplan-Meier curves showed that HCC patients with higher SOX9 expression had significantly lower 5-year survival rate, while patients with higher expression of DCN or DPT had higher 5-year survival rates.
