Basic Study
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World J Gastrointest Oncol. Mar 15, 2022; 14(3): 664-677
Published online Mar 15, 2022. doi: 10.4251/wjgo.v14.i3.664
O6-methylguanine DNA methyltransferase is upregulated in malignant transformation of gastric epithelial cells via its gene promoter DNA hypomethylation
Yue-Xia Chen, Lu-Lu He, Xue-Ping Xiang, Jing Shen, Hong-Yan Qi
Yue-Xia Chen, Lu-Lu He, Hong-Yan Qi, Department of Pathology and Pathophysiology and Department of Radiation Oncology of the Second Affiliated Hospital, University School of Medicine, Zhejiang University, Hangzhou 310058, Zhejiang Province, China
Yue-Xia Chen, Department of Pathology, Third Hospital of Nanchang, Nanchang 330000, Jiangxi Province, China
Xue-Ping Xiang, Department of Pathology, the Second Affiliated Hospital, Zhejiang University, Hangzhou 310058, Zhejiang Province, China
Jing Shen, Department of Pathology and Pathophysiology andDepartment of Medical Oncology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310058, Zhejiang Province, China
Author contributions: Chen YX and Lulu He contributed to the acquisition and analyses of the data; Xiang XP contributed to the collection and immunostaining of clinical samples; Shen J and Qi HY designed the study and made the critical revisions of the manuscript.
Supported by National Natural Science Foundation of China, No. 81472543 and No. 81772919; Zhejiang Provincial Natural Science Foundation of China, No. LY18H160024 and No. LY20H160040.
Institutional review board statement: The study was approved by the ethics committee of Zhejiang University School of Medicine (No. 2017026).
Institutional animal care and use committee statement: The work described herein was approved by the Laboratory Animals Welfare Ethics Review Committee of Zhejiang University (No. ZJU20170522).
Conflict-of-interest statement: The authors declare no conflicts of interest for this article.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hong-Yan Qi, PhD, Associate Professor, Department of Pathology and Pathophysiology and Department of Radiation Oncology of the Second Affiliated Hospital, University School of Medicine, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, Zhejiang Province, China. qihongyan@zju.edu.cn
Received: July 27, 2021
Peer-review started: July 27, 2021
First decision: October 3, 2021
Revised: November 10, 2021
Accepted: February 10, 2022
Article in press: February 10, 2022
Published online: March 15, 2022
Processing time: 225 Days and 21.4 Hours
Core Tip

Core Tip: This study revealed a molecular regulatory mechanism of O6-methylguanine-DNA methyltransferase (MGMT) gene upregulation in the early stage of tumor development, and improved the understanding of the dynamic change of MGMT expression in different stages of tumor development, providing a new entry point for further study of the expression mechanism of key regulatory genes in the process of chemical carcinogenesis.