Basic Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Nov 15, 2021; 13(11): 1709-1724
Published online Nov 15, 2021. doi: 10.4251/wjgo.v13.i11.1709
Genome-wide CRISPR-Cas9 screening identifies that hypoxia-inducible factor-1a-induced CBX8 transcription promotes pancreatic cancer progression via IRS1/AKT axis
Bu-Wei Teng, Kun-Dong Zhang, Yu-Han Yang, Zeng-Ya Guo, Wei-Wei Chen, Zheng-Jun Qiu
Bu-Wei Teng, Zheng-Jun Qiu, Shanghai General Hospital of Nanjing Medical University, 100 Haining Road, Shanghai 200080, China
Bu-Wei Teng, Lianyungang Clinical College of Nanjing Medical University/The First People’s Hospital of Lianyungang, 6 Zhenhua East Road, Haizhou District, City of Lianyungang, Jiangsu Province 222061, China
Kun-Dong Zhang, Yu-Han Yang, Zeng-Ya Guo, Wei-Wei Chen, Department of General Surgery, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai 200080, China
Author contributions: Qiu ZJ and Teng BW conceived and supervised the study; Teng BW performed most of the experiments and data analysis; Zhang KD performed most functional experiments and data analysis; Qiu ZJ and Teng BW wrote the manuscript; Yang YH, Guo ZY, and Chen WW took part in the discussions; all the authors discussed the results and commented on the manuscript, and approved the final version.
Supported by National Natural Science Foundation of China (General Program), No. 81974372 (to Qiu ZJ).
Institutional review board statement: The study was reviewed and approved by the Shanghai General Hospital Institutional Review Board (Approval No. 2020KY083).
Conflict-of-interest statement: The authors declare no conflicts of interest for this work.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Zheng-Jun Qiu, MD, Chief Doctor, Director, Surgeon, Shanghai General Hospital of Nanjing Medical University; Department of General Surgery, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, No. 100 Haining Road, Shanghai 201600, China. qiuzjdoctor@sina.com
Received: April 14, 2021
Peer-review started: April 20, 2021
First decision: June 23, 2021
Revised: June 24, 2021
Accepted: August 23, 2021
Article in press: August 23, 2021
Published online: November 15, 2021
Core Tip

Core Tip: The authors demonstrated that hypoxia-inducible factor-1a induced chromobox (CBX)8 transcription by binding to the promoter of CBX8. CBX8 efficiently activated the PI3K/AKT signaling by upregulating insulin receptor substrate 1. The newly identified signaling axis may support the development of new therapeutic strategies for pancreatic cancer.