Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Jan 15, 2020; 12(1): 54-65
Published online Jan 15, 2020. doi: 10.4251/wjgo.v12.i1.54
Identification of candidate biomarkers correlated with pathogenesis of postoperative peritoneal adhesion by using microarray analysis
Yao-Yao Bian, Li-Li Yang, Yan Yan, Min Zhao, Yan-Qi Chen, Ya-Qi Zhou, Zi-Xin Wang, Wen-Lin Li, Li Zeng
Yao-Yao Bian, Ya-Qi Zhou, Zi-Xin Wang, School of Nursing, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China
Li-Li Yang, Min Zhao, Yan-Qi Chen, Li Zeng, School of First Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China
Li-Li Yang, Wen-Lin Li, Li Zeng, Jingwen Library, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China
Yan Yan, Guang′anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China
Author contributions: Bian YY, Yang LL, and Yan Y contributed equally to this study; Bian YY conceived and designed the study, made the data acquisition, and prepared the manuscript with Yang LL; Yan Y and Zhao M conducted the data analysis; Chen YQ, Zhou YQ, and Wang ZX contributed to the animal experiment; Li WL and Zeng L provided several suggestions for manuscript revision.
Supported by the National Natural Science Foundation of China, No. 81704084, No. 81603529, and No. 81673982; the Science and Technology Projects of Jiangsu Provincial Bureau of Traditional Chinese Medicine, No. YB2017002 and No. YB2015002; the Natural Science Foundation of the Jiangsu Higher Education Institutions, No. 16KJB360002; the Postgraduate Research and Practice Innovation Program of Jiangsu Province, No. KYCX18_1541; the Qing Lan Project; and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Open Projects of the Discipline of Chinese Medicine of Nanjing University of Chinese Medicine (ZYX03KF63), Jiangsu Government Scholarship for Overseas Studies and China Scholarship Council.
Institutional animal care and use committee statement: The protocol was approved by the Laboratory Animal Management Committee of the Nanjing University of Chinese Medicine.
Conflict-of-interest statement: The authors have no conflicts of interest to disclose.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Li Zeng, PhD, Professor, School of First Clinical Medicine and Jingwen Library, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, Jiangsu Province, China. zengli@njucm.edu.cn
Received: June 4, 2019
Peer-review started: June 4, 2019
First decision: July 31, 2019
Revised: August 5, 2019
Accepted: September 12, 2019
Article in press: September 12, 2019
Published online: January 15, 2020
Processing time: 213 Days and 21.5 Hours
Core Tip

Core tip: Postoperative peritoneal adhesion remains an urgent clinical concern due to increasing abdominal surgery. The occurrence and formation of adhesion are from complex biological processes. However, the molecular mechanisms underlying the basis of microarray data profile, followed by peritoneal adhesion formation, are largely unknown. In this study, we uncovered the underlying pathogenesis of postoperative peritoneal adhesion at the molecular level using bioinformatics analysis methods. The results were further validated using animal experiments. It showed that the regulatory evidence chain of TLR4/MyD88/NF-κB/inflammatory cytokines/peritoneal adhesion played key roles in the pathogenesis of postoperative adhesion. Our findings may provide new insights into peritoneal adhesion formation.