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©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Feb 15, 2023; 15(2): 318-331
Published online Feb 15, 2023. doi: 10.4251/wjgo.v15.i2.318
microRNA-627-5p inhibits colorectal cancer cell proliferation, migration and invasion by targeting Wnt2
Dong-Yan Zhao, Teng-Fei Yin, Xi-Zhen Sun, Yuan-Chen Zhou, Qian-Qian Wang, Ge-Yujia Zhou, Shu-Kun Yao
Dong-Yan Zhao, School of Biology & Basic Medical Sciences, Soochow University, Soochow 215213, Jiangsu Province, China
Teng-Fei Yin, Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China
Xi-Zhen Sun, Department of Gastroenterology, Beijing Jishuitan Hospital, Beijing 100035, China
Yuan-Chen Zhou, Qian-Qian Wang, Ge-Yujia Zhou, Shu-Kun Yao, Department of Gastroenterology, China-Japan Friendship Hospital, Beijing 100029, China
Yuan-Chen Zhou, Qian-Qian Wang, Graduate School, Peking University China-Japan Friendship School of Clinical Medicine, Beijing 100029, China
Ge-Yujia Zhou, Graduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100029, China
Author contributions: Zhao DY designed and performed the study, analyzed the data, and drafted the manuscript; Yin TF and Sun XZ collected colorectal samples from subjects, and provided guidance on experimental procedures; Zhou YC, Wang QQ, and Zhou GYJ collected the clinical data and colorectal samples from the subjects; Yao SK designed the study, supervised the study performance, revised the manuscript, and obtained the funding; all authors read and approved the final manuscript.
Supported by the National Key Development Plan for Precision Medicine Research, No. 2017YFC0910002.
Institutional review board statement: This study was reviewed and approved by the Ethics Committee of China-Japan Friendship Hospital (Approval No. 2018-116-K85).
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: There are no conflicts of interest to report.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
https://creativecommons.org/Licenses/by-nc/4.0/ Corresponding author: Shu-Kun Yao, Doctor, MD, PhD, Chief Physician, Professor, Department of Gastroenterology, China-Japan Friendship Hospital, No. 2 Yinghua East Road, Chaoyang District, Beijing 100029, China. shukunyao@126.com
Received: September 22, 2022
Peer-review started: September 22, 2022
First decision: October 21, 2022
Revised: November 4, 2022
Accepted: December 30, 2022
Article in press: December 30, 2022
Published online: February 15, 2023
ARTICLE HIGHLIGHTS
Research background
Population aging has given rise to the incidence rate of colorectal cancer (CRC) worldwide. Better elucidation of the mechanisms underlying the formation and growth of CRC is very helpful for the development of new therapy.
Research motivation
Latest studies have shown that miRNAs generally regulate the expression of oncogenes or tumour suppressor genes and exert integral roles in modulating cancer-related pathways and mediating the formation and progression of CRC. However, whether miR-627-5p is involved in the tumorigenesis of colorectal tumours or not is largely unexplored.
Research objectives
This current study is designed to verify the function of miR-627-5p in colorectal tumorigenesis by targeting Wnt2/β-catenin signalling pathway.
Research methods
The levels of miR-627-5p and Wnt2 were detected in CRC tissues. Functional experiments, including CCK-8, flow cytometry, Matrigel invasion, and scratch assays, were conducted to elucidate the function of miR-627-5p and wnt2 in colorectal tumour cells. Dual luciferase reporter tests were carried out to investigate how miR-627-5p and Wnt2 interact. The critical signalling pathway modulated by miR-627-5p was further identified.
Research results
Wnt2 transcript expression was markedly increased in colorectal tumour tissues and negatively correlated with miR-627-5p level. Upregualtion of miR-627-5p inhibited cancer cells’ abilities to invade growth and migrate by directly restraining Wnt2 expressions. Furthermore, miR-627-5p exerted the suppressive role in CRC via inactivating the Wnt2/β-catenin signalling.
Research conclusions
miR-627-5p restrained the malignant biological properties of CRC cells via directly inhibiting Wnt2 expression to modulate the classical Wnt/β-catenin signalling.
Research perspectives
miRNA-627-5p/Wnt2/β-catenin may have potential therapeutic application for CRC.
