Copyright
©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
Direct interaction between Rab5a and Rab4a enhanced epidermal growth factor-stimulated proliferation of gastric cancer cells
Guo-Jun Cao, Di Wang, Zhao-Pei Zeng, Guo-Xiang Wang, Chun-Jiu Hu, Zhi-Fang Xing
Guo-Jun Cao, Di Wang, Department of Laboratory Medicine, Huashan Hospital North, Shanghai Medical College, Fudan University, Shanghai 201907, China
Di Wang, School of Life Sciences, Fudan University, Shanghai 200433, China
Zhao-Pei Zeng, Department of Laboratory Medicine, Diniu (Shanghai) Health Technology Co., Shanghai 201703, China
Guo-Xiang Wang, Department of Neurology, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institute of Biological Science, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Chun-Jiu Hu, Department of Gastroenterology, Ningbo First Hospital, Ningbo 315000, Zhejiang Province, China
Zhi-Fang Xing, Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China
Author contributions: Cao GJ and Wang D contributed equally to this work and are joint first authors; Xing ZF and Wang GX contributed equally to the supervision of the study and share co-senior authorship; Cao GJ carried out western blot and co-immunoprecipitation experiments and helped with the experimental design; Wang D was the main writer of this manuscript and took part in the planning and execution of the immunostaining and co-immunoprecipitation experiments; Zeng ZP also wrote the main part of the manuscript and took part in the analysis of the data; Hu CJ participated in the coordination of the study and reviewed the manuscript; Wang GX contributed to experimental design and data analysis; Xing ZF helped with the experimental design and implementation of this research.
Supported by The Shanghai Health and Family Planning Commission, No. 20154Y0141; Shanghai "Rising Stars of Medical Talent" Youth Development Program (Youth Medical Talents – Clinical Laboratory Practitioners Program); and the Project of Huashan Hospital North, Fudan University, No. HSBY2019020.
Institutional review board statement: The study was reviewed and approved by the Minhang Center Hospital, Fudan University Institutional Review Board (2015 No. 42).
Conflict-of-interest statement: We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product.
Data sharing statement: No additional unpublished data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
http://creativecommons.org/Licenses/by-nc/4.0/ Corresponding author: Zhi-Fang Xing, Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, No. 12 Middle Urumqi Road, Shanghai 200040, China. xzfisme@163.com
Received: January 28, 2021
Peer-review started: January 28, 2021
First decision: June 4, 2021
Revised: June 16, 2021
Accepted: July 14, 2021
Article in press: July 14, 2021
Published online: October 15, 2021
ARTICLE HIGHLIGHTS
Research background
Gastric cancer (GC) is a common malignant tumor of digestive system with a poor overall prognosis.
Research motivation
The purpose of our study is to explore the pathogenesis of GC and improve the prognosis of GC.
Research objectives
We constructed HGC-27 cell lines overexpressing green fluorescent protein-Rab5a or red fluorescent protein-Rab4a and tissue samples of 42 patients with GC.
Research methods
Western blot, co-immunoprecipitation, immunohistochemistry, immunoprecipitation assay, cell culture, confocal microscopy, etc. were utilized.
Research results
Rab5a and Rab4a were overexpressed in GC tissues. The direct interaction between Rab5a and Rab4a enhanced epidermal growth factor-stimulated proliferation of GC cells.
Research conclusions
Co-overexpression of Rab5a and Rab4a in GC may promote the endosomal recycling of epidermal growth factor receptor, which in turn contributes to poor prognosis and tumor progression in GC patients. Inhibition of Rab5a or Rab4a expression might be a promising therapy for refractory GC.
Research perspectives
To explore the pathogenesis of GC and improve the prognosis of GC.
