Observational Study
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World J Gastrointest Oncol. Dec 15, 2014; 6(12): 444-449
Published online Dec 15, 2014. doi: 10.4251/wjgo.v6.i12.444
TT genotype of GNAS1 T393C polymorphism predicts better outcome of advanced non-small cell lung cancer patients
Hong-Yun Gong, Wei-Guo Hu, Xiu-Ling Wang, Fan Zhu, Qin-Bin Song
Hong-Yun Gong, Wei-Guo Hu, Qin-Bin Song, Department of Oncology, Renmin Hospital of Wuhan University, Wuhan 430060, Hebei Province, China
Xiu-Ling Wang, Fan Zhu, Department of Medical Microbiology, School of Medicine, Wuhan University, Wuhan 430060, Hebei Province, China
Author contributions: Gong HY searched the literature and isolated genomic DNA from peripheral blood leucocytes; Hu WG collected blood samples from patients; Wang XL performed the genotyping of genomic DNA; Zhu F gave suggestions in writing the article; Song QB directed and coordinated the study; all authors were involved in organizing and refining the article.
Correspondence to: Dr. Qin-Bin Song, Department of Oncology, Renmin Hospital of Wuhan University, No. 238 Jiefang Road, Wuhan 430060, Hebei Province, China. baxinfangkaihao@sina.com
Telephone: +86-027-88041911
Received: March 3, 2014
Revised: October 28, 2014
Accepted: October 31, 2014
Published online: December 15, 2014
Abstract

AIM: To evaluate the potential prognostic value of GNAS1 T393C polymorphism in advanced non-small cell lung cancer.

METHODS: We extracted genomic DNA from the peripheral blood leucocytes of 94 patients with advanced non-small cell lung cancer. Quantitative real-time polymerase chain reaction was used to determine the allelic discrimination. The correlation between genotype and overall survival was evaluated using the multivariate analysis and Kaplan-Meier approach.

RESULTS: Thirty-eight out of 94 (40%) patients displayed a TT genotype, 29 out of 94 (31%) a CT genotype and 27 out of 94 (29%) a CC genotype. The median survival of TT (25 mo) genotype carriers was longer than CT (12 mo) or CC (8 mo) genotype carriers. The favorable TT genotype predicted better overall survival (OS) (2-year OS: 48%; P =0.01) compared with CT (2-year OS: 18%) or CC (2-year OS: 15%) genotype. However, dichotomization between C-genotypes (CC + CT) and T-genotypes (TT) revealed significantly lower survival rates (2-year OS: 16%; P = 0.01) for C allele carriers.

CONCLUSION: Our data provided strong evidence that the GNAS1 T393C genetic polymorphism influenced the prognosis in advanced non-small lung cancer with a worse outcome for C allele carriers.

Keywords: GNAS1, Polymorphism, Advanced non-small cell lung cancer, Prognosis

Core tip: We genotyped GNAS1 T393C single nucleotide polymorphism in a homogenous (Han) study population of patients to evaluate the effect of this polymorphism on survival in non-small cell lung cancer (NSCLC). Our study indicated that the GNAS1 T393C polymorphism affected the overall survival in advanced NSCLC with a worse outcome for C allele carriers.