Published online Jul 15, 2024. doi: 10.4251/wjgo.v16.i7.3158
Revised: April 25, 2024
Accepted: May 17, 2024
Published online: July 15, 2024
Processing time: 210 Days and 13 Hours
Chronic atrophic gastritis (CAG) is a prevalent chronic gastritis usually accom
To investigate the pharmacological effects of Yiqi Jiedu Huayu decoction (YJHD) on precancerous lesions of CAG.
A CAG rat model was established by Helicobacter pylori bacteria solution combined with N-methyl-N’-nitro-N-nitrosoguanidine. Histopathological measurements were conducted by hematoxylin-eosin and alcian blue and periodic acid-Schiff staining. Serum levels of inflammatory factors and gastric mucosal-related factors were examined using enzyme-linked immunosorbent assay. Protein and mRNA levels were quantified via western blot and quantitative real-time polymerase chain reaction analysis, respectively. Molecular interaction was verified by chromatin immunoprecipitation (ChIP) assay.
YJHD greatly attenuated pathological changes in the gastric mucosa and precancerous lesions in CAG rats. Meanwhile, YJHD treatment reduced serum levels of inflammatory factors [interleukin (IL)-6, tumor necrosis factor-α and C-reactive protein] and increased serum levels of gastric mucosal-related factors (gastrin, pepsin, somatostatin and prostaglandin E2) in CAG rats. In addition, YJHD administration suppressed NLRP3 inflammasome-mediated cell pyroptosis, as well as the activation of TLR4/NF-κB and IL-6/STAT3 signaling pathways. Mechanically, ChIP experiments confirmed that NLRP3 transcription was regulated by TLR4/NF-κB and IL-6/STAT3 signaling.
Taken together, YJHD alleviated NLRP3 inflammasome formation and pyroptosis of epithelial cells in CAG, potentially through the inactivation of TLR4/NF-κB and IL-6/STAT3 pathways.
Core Tip: Yiqi Jiedu Huayu decoction (YJHD), an empirical formula comprising multiple traditional Chinese medicines, has demonstrated efficacy in preventing and treatment of human gastrointestinal tumors. However, its impact on precancerous lesion of gastric cancer (PLGC) remains poorly understood, making this study significant. Here, we found that YJHD treatment markedly inhibited PLGC. Additionally, our experiments indicated that YJHD inhibited NLRP3 inflammasome formation and pyroptosis of epithelial cells in chronic atrophic gastritis by deactivating the TLR4/NF-κB and IL-6/STAT3 pathways.