Lu Y, Wang XM, Li ZS, Wu AJ, Cheng WX. Hsa_circ_0001658 accelerates the progression of colorectal cancer through miR-590-5p/METTL3 regulatory axis. World J Gastrointest Oncol 2023; 15(1): 76-89 [PMID: 36684043 DOI: 10.4251/wjgo.v15.i1.76]
Corresponding Author of This Article
Yang Lu, MMed, Chief Physician, Department of Oncology, PKUCare Luzhong Hospital, No. 65 Taigong Road, Linzi District, Zibo 255400, Shandong Province, China. ly76730@163.com
Research Domain of This Article
Oncology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastrointest Oncol. Jan 15, 2023; 15(1): 76-89 Published online Jan 15, 2023. doi: 10.4251/wjgo.v15.i1.76
Hsa_circ_0001658 accelerates the progression of colorectal cancer through miR-590-5p/METTL3 regulatory axis
Yang Lu, Xing-Ming Wang, Ze-Shu Li, Ai-Juan Wu, Wen-Xia Cheng
Yang Lu, Xing-Ming Wang, Ze-Shu Li, Ai-Juan Wu, Department of Oncology, PKUCare Luzhong Hospital, Zibo 255400, Shandong Province, China
Wen-Xia Cheng, Department of Oncology, Zibo Maternal and Child Health Hospital, Zibo 255095, Shandong Province, China
Author contributions: Lu Y designed the study, and wrote the manuscript; Lu Y, Wang X, and Li Z performed the research and collected data; Wu A and Cheng W contributed to the analysis and editing of the manuscript; All authors have read and approved the final manuscript.
Institutional review board statement: This study was approved by the Ethics Committee of the PKUCare Luzhong Hospital (Approval No. 2020-L259).
Informed consent statement: The patients provided the informed written consent, and the resected pathological tissues were allowed to be used for pathological examination and biomedical research.
Conflict-of-interest statement: All authors have no conflicts of interest to declare.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: This study was not involved any animal experiments.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Yang Lu, MMed, Chief Physician, Department of Oncology, PKUCare Luzhong Hospital, No. 65 Taigong Road, Linzi District, Zibo 255400, Shandong Province, China. ly76730@163.com
Received: September 28, 2022 Peer-review started: September 28, 2022 First decision: October 21, 2022 Revised: November 1, 2022 Accepted: December 21, 2022 Article in press: December 21, 2022 Published online: January 15, 2023 Processing time: 103 Days and 22 Hours
Abstract
BACKGROUND
As reported, multiple circular RNAs (circRNAs) interfere with colorectal cancer (CRC) progression. Here, circRNA_0001658 (circ_0001658) is focused on studying how it works in CRC.
AIM
Clarify the expression pattern, biological function, and underlying mechanism of circ_0001658 of CRC tumorigenesis.
METHODS
In CRC-related chip data retrieved using the database named Gene Expression Omnibus, different expressions of circRNAs between CRC and normal tissue samples were identified. Quantitative Real-time PCR and Western blot ensured the analysis on circ_0001658, microRNA-590-5P (miR-590-5p), and methyltransferase-like 3 (METTL3) mRNA expressions in tissues and cells. Cell counting kit-8 and flow cytometry were used to detect cell proliferation, apoptosis and migration. The targeting relations between circ_0001658, miR-590-5p, and METTL3 mRNA 3'-untranslated region were under the verification of bioinformatics prediction and dual luciferase-based reporter gene assays. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were employed on the downstream targets of miR-590-5p using the Database for Annotation, Visualization and Integrated Discovery database.
RESULTS
Circ_0001658 and METTL3 mRNA was elevated in CRC tissues and cells, whereas miR-590-5p was decreased. Circ_0001658 overexpression promoted the proliferation of HT29 cells, inhibited apoptosis, and accelerated the cell cycle. In SW480 cells, knocking down circ_0001658 had the opposite effect. Circ_0001658 could specifically bind to miR-590-5p and negatively modulate its expressions; METTL3 is a miR-590-5p target that can be positively regulated by circ 0001658. Circ 0001658 was inversely associated with miR-590-5p expression while positively with METTL3 expressions.
CONCLUSION
Circ_0001658 regulates the miR-590-5p/METTL 3-axis to increase CRC cell growth and decrease apoptosis.
Core Tip: As we know, the progression of colorectal cancer (CRC) is significantly influenced by circular RNAs (circRNAs). This study focused on circRNA_0001658 (circ_0001658) and delved into how it works in CRC. The results confirmed that circ_0001658 and methyltransferase-like 3 (METTL3) mRNA expression in CRC tissues and cells were increased, while miR-590-5p was decreased. Circ_0001658 was inversely associated with miR-590-5p expression while positively with METTL3 expressions. In a word, circ_0001658 accelerates the progression of CRC through miR-590-5p/METTL3 regulatory axis.
