Zhang ZY, Guo S, Zhao R, Ji ZP, Zhuang ZN. Clinical significance of SQSTM1/P62 and nuclear factor-κB expression in pancreatic carcinoma. World J Gastrointest Oncol 2020; 12(7): 719-731 [PMID: 32864040 DOI: 10.4251/wjgo.v12.i7.719]
Corresponding Author of This Article
Zhuo-Nan Zhuang, MD, Associate Chief Physician, Department of Gastrointestinal Surgery, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, No. 168, Litang Road, Tiantongyuan, Changping District, Beijing 102218, China. 1054766178@qq.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastrointest Oncol. Jul 15, 2020; 12(7): 719-731 Published online Jul 15, 2020. doi: 10.4251/wjgo.v12.i7.719
Clinical significance of SQSTM1/P62 and nuclear factor-κB expression in pancreatic carcinoma
Zhao-Yang Zhang, Sen Guo, Rui Zhao, Zhi-Peng Ji, Zhuo-Nan Zhuang
Zhao-Yang Zhang, Department of Emergency Surgery, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China
Sen Guo, Rui Zhao, Department of General Surgery, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China
Zhi-Peng Ji, Department of General Surgery, Second Affiliated Hospital of Shandong University, Jinan 250033, Shandong Province, China
Zhuo-Nan Zhuang, Department of Gastrointestinal Surgery, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing 102200, China
Author contributions: Zhang ZY and Zhuang ZN were major contributors in writing the manuscript and analyzing the data; Guo S and Zhuang ZN made substantial contributions to study design; Guo S, Zhao R, and Ji ZP were major contributors in doing the animal experiments; all authors read and approved the final manuscript.
Institutional review board statement: The study was reviewed and approved by the Ethics Committee of Qilu Hospital, Shandong University.
Conflict-of-interest statement: Dr. Zhuang reports grants from Excellent-Talent Training Project of Beijing Foundation (No. 12016B4023) and from the Natural Sciences Foundation of Shandong Province (No. ZR2014HM101), during the conduct of the study. All other authors have nothing to disclose.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Zhuo-Nan Zhuang, MD, Associate Chief Physician, Department of Gastrointestinal Surgery, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, No. 168, Litang Road, Tiantongyuan, Changping District, Beijing 102218, China. 1054766178@qq.com
Received: March 25, 2020 Peer-review started: March 25, 2020 First decision: April 26, 2020 Revised: May 3, 2020 Accepted: May 27, 2020 Article in press: May 27, 2020 Published online: July 15, 2020 Processing time: 111 Days and 22.8 Hours
Abstract
BACKGROUND
Overexpression of SQSTM1 (sequestosome 1, P62) and nuclear factor-κB (NF-κB) plays an important role in the invasion and metastasis of a variety of malignant tumors.
AIM
To explore the expression of P62 and NF-κB in pancreatic cancer and their relationship with clinicopathological features.
METHODS
The expression levels of P62 and NF-κB were analyzed by immunohistochemistry with a tissue chip containing 40 cases of human pancreatic carcinoma. Then we analyzed the correlation among P62 expression, phospho-P65 expression, and clinicopathological features of pancreatic carcinoma samples.
RESULTS
P62 expression was mainly observed in the cytoplasm of pancreatic carcinoma cells. Phosphorylated P65 (phospho-P65) was mainly expressed in the nucleus and cytoplasm of pancreatic carcinoma cells. There was a significant difference in P62 expression among T stages. And a significant difference in phosphor-P65 expression among pathology types was noted. In the cases with strongly positive P62 expression, significant differences were found in age. And there were significant differences in T stage and tumor-node-metastasis stage in the cases with strongly positive phosphor-P65 expression.
CONCLUSION
In pancreatic carcinoma, P62 expression is significantly correlated with T stage. It may be a valuable malignant indicator for human pancreatic carcinoma.
Core Tip: SQSTM1 (sequestosome 1, P62) and nuclear factor-κB play an important role in the invasion and metastasis of a variety of malignant tumors. We discovered that P62 expression can be used as a valuable indicator for the malignancy of human pancreatic carcinoma.
