Copyright
©2014 Baishideng Publishing Group Inc.
World J Stem Cells. Jul 26, 2014; 6(3): 288-295
Published online Jul 26, 2014. doi: 10.4252/wjsc.v6.i3.288
Published online Jul 26, 2014. doi: 10.4252/wjsc.v6.i3.288
Ref. | Osteogenic medium | Culturing condition | Analytic methods | Outcome of osteogenic ability |
Park et al[22] | 0.1 mmol/L nonessential ammino acids, 50 μg/mL ascorbic acid-2-phosphate, 100 nmol/L dexamethasone and 10 mmol/L β-glycerolphosphate | Mechanical stimulation by dynamic hydraulic compression | AP, Alizarin red stian, qPCR, immunofluorescence | BMSC > ASC under mechanical stimulation |
Vishnubalaji et al[20] | 10 nmol/L calcitriol, 10 nmol/L β-glycerophosphate, 50 μg/mL L-ascorbic acid, 10 nmol/L dexamethasone | 2-D static cuture | AP, Alizarin red, Von Kossa stain, Calcium concentration, qPCR | BMSC > ASC |
De Ugarte et al[16] | 50 μg/mL ascorbic acid-2-phosphate, 100 nmol/L dexamethasone and 10 mmol/L β-glycerolphosphate | 2-D static culture | AP activity, calcium assay | BMSC = ASC |
Im et al[17] | 100 nmol/L dexamethasone, 50 μmol/L ascorbate-2- phosphate, 10 mmol/L dexamethasone β-glycerophosphate | 2D static culture | AP activity, Von Kossa staining | BMSC > ASC |
Shafiee et al[19] | 10 nmol/L dexamethason, 0.2 mmol/L ascorbic acid-2-phosphate, 10 mmol/L β-glycerophosphate | 2D cell culture | AP activity, Alizarin red staining, qPCR | BMSC > ASC |
Liu et al[18] | 0.1 μmol/L dexamethasone, 50 μmol/L ascorbic acid-2-phosphate and 10 mmol/L β-glycerophosphate | 2D cell culture | Alizarin red stain and microarray | BMSC > ASC |
Zhang et al[21] | 10 mmol/L β-glycerophosphate, 10-8 mol/L dexamethasone, and 0.2 mmol/L ascorbic acid | 2D cell culture 3D cell culture | Calcium assay, qPCR, SEM | BMSC > ASC |
Ref. | Animal model | Scaffold | Analytic methods | Outcome of osteogenic ability |
Hayashi et al[27] | Subcutaneous implantation in rat | Hydroxyapatite | Micro-CT | New bone volume BMSC (6.85 ± 1.89 mm3) > ASC (0.05 ± 0.05 mm3) |
Niemeyer et al[32] | 3-cm tibial defect in sheep | Collagen sponge | Radiographic and histologic analysis | BMSC > ASC BMSC = ASC + PRP No original data for bone volume |
Kang et al[23] | 1.5-cm radial bone defect in dog | Tricalcium phosphate | Radiographic, histological and histomorphometric analysis | New bone percentage: BMSC (33.56%) = ASC (33.9%) |
Stockmann et al[33] | 1-cm calvarial bone defect in pig | Bovine collagen type I | Microradiography, histomorphometric evaluation | BMSC = ASC No original data for bone volume |
Wen et al[34] | 5-mm calvarial defect in SD rat | Collagen gel | X-ray and histology | BMSC = ASC No original data for bone volume |
Zhang et al[21] | Subcutaneous pocket of nude mice | PCL/TCP | Micro-CT | New bone volume: BMSC (16.6 ± 3.0 mm3) > ASC (9.1 ± 1.1 mm3) |
- Citation: Liao HT, Chen CT. Osteogenic potential: Comparison between bone marrow and adipose-derived mesenchymal stem cells. World J Stem Cells 2014; 6(3): 288-295
- URL: https://www.wjgnet.com/1948-0210/full/v6/i3/288.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v6.i3.288