Copyright
©2013 Baishideng Publishing Group Co.
World J Stem Cells. Oct 26, 2013; 5(4): 229-237
Published online Oct 26, 2013. doi: 10.4252/wjsc.v5.i4.229
Published online Oct 26, 2013. doi: 10.4252/wjsc.v5.i4.229
Table 1 Primary and secondary antibodies used in the study
Antibody | Specie | Supplier | Dilution | |
Primary antibody | ||||
β-III-Tubulin (TuJ-1) | Mouse | R and D | 1:1000 | |
GFAP | Rabbit | Eurodiagnostic | 1:100 | |
MAP2 | Rabbit | S.Cruz | 1:250 | |
6E10 | Mouse | Covance | 1:1000 | |
CNPase | Mouse | Chemicon | 1:250 | |
Secondary antibody | ||||
Anti-Mo RRX | Donkey | Alexa | 1:600 | |
Anti-Mo Cy2 | Donkey | Jackson | 1:100 | |
Anti-Rb Cy2 | Donkey | Jackson | 1:100 | |
Anti-Rb RRX | Donkey | Jackson | 1:100 |
Table 2 Genotype-dependent cell yield per animal
Wild type | Tg2576 | |
Primary neurospheres | 1.7 × 105± 5 × 104 | 1.7 × 105± 1×104 |
Secondary neurospheres | 1.7 × 105± 1.4× 104 | 1.3×105± 9.7×103a |
Table 3 Polymerase chain reaction array analysis
Gene | Regulation |
Acetylcholinesterase | 3.41 |
Amyloid beta (A4) precursor protein-binding, family B, member 1 | 2.33 |
CDK5 regulatory subunit associated protein 1 | 2.07 |
Dopamine receptor D2 | 2.62 |
V-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) | 2.17 |
Glial cell line derived neurotrophic factor | 3.13 |
Interleukin 3 | 2.69 |
Notch gene homolog 2 (Drosophila) | 2.29 |
Odd Oz/ten-m homolog 1 (Drosophila) | 3.81 |
Par-3 (partitioning defective 3) homolog (C. elegans) | 2.39 |
Vascular endothelial growth factor A | 3.01 |
- Citation: Baldassarro VA, Lizzo G, Paradisi M, Fernández M, Giardino L, Calzà L. Neural stem cells isolated from amyloid precursor protein-mutated mice for drug discovery. World J Stem Cells 2013; 5(4): 229-237
- URL: https://www.wjgnet.com/1948-0210/full/v5/i4/229.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v5.i4.229