Effects of interleukin-10 treated macrophages on bone marrow mesenchymal stem cells via signal transducer and activator of transcription 3 pathway

Table 1 The real-time fluorescence quantitative polymerase chain reaction primer sequences for macrophage expression detection

Primer	Sequences
Actb	Forward: 5’-GGAGATTACTGCCCTGGCTCCTAGC-3’
	Reverse: 5’-GGCCGGACTCATCGTACTCCTGCTT-3’
Gapdh	Forward: 5’-TATGACTCTACCCACGGCAA-3’
	Reverse: 5’-ATACTCAGCACCAGCATCACC-3’
Arg1	Forward: 5’-AGTGTGGTGCTGGGTGGAGAC-3’
	Reverse: 5’-GCGGAGTGTTGATGTCAGTGTGAG-3’
Il10	Forward: 5’-CGCATCCAGACACACACAGACTAG-3’
	Reverse: 5’-GCCCAGAGACAGACAAGCAAGAG-3’
Inos	Forward: 5’-GAGACGCACAGGCAGAGGTTG-3’
	Reverse: 5’-AGCAGGCACACGCAATGATG G-3’
Tnfa	Forward: 5’-ATGGGCTCCCTCTCATCAGT-3’
	Reverse: 5’-GCTTGGTGGTTTGCTACGAC-3’

Relative mRNA expression levels were analyzed using the 2^-∆∆Ct method with glyceraldehyde-3-phosphate dehydrogenase and β-actin as internal references. Arg1: Arginase 1; Il10: Interleukin-10; Inos: Inducible nitric oxide synthase (gene); Tnfa: Tumor necrosis factor-α; Gapdh: Glyceraldehyde-3-phosphate dehydrogenase; Actb: β-actin.

Table 2 The real-time fluorescence quantitative polymerase chain reaction primer sequences for differentiation of bone marrow mesenchymal stem cells detection

Primer	Sequences
Gapdh	Forward: 5’-TATGACTCTACCCACGGCAA-3’
	Reverse: 5’-ATACTCAGCACCAGCATCACC-3’
Alp	Forward: 5’-AACGTGGCCAAGAACATCATCA-3’
	Reverse: 5’-TGTCCATCTCCAGCCGTGTC-3’
Ocn	Forward: 5’-AAAGCCCAGCGACTCT-3’
	Reverse: 5’-CTAAACGGTGGTGCCATAGAT-3’
Runx2	Forward: 5’-GCTTCTCCAACCCACGAATG-3’
	Reverse: 5’-GAACTGATAGGACGCTGACGA-3’

Relative mRNA expression levels were analyzed using the 2^-∆∆Ct method with glyceraldehyde-3-phosphate dehydrogenase as an internal reference. Alp: Alkaline phosphatase; Ocn: Osteocalcin; Runx2: Recombinant runt related transcription factor 2; Gapdh: Glyceraldehyde-3-phosphate dehydrogenase.