Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Jul 26, 2017; 9(7): 98-106
Published online Jul 26, 2017. doi: 10.4252/wjsc.v9.i7.98
Modifying oxygen tension affects bone marrow stromal cell osteogenesis for regenerative medicine
Yusuke Inagaki, Manabu Akahane, Takamasa Shimizu, Kazuya Inoue, Takuya Egawa, Tsutomu Kira, Munehiro Ogawa, Kenji Kawate, Yasuhito Tanaka
Yusuke Inagaki, Takamasa Shimizu, Kazuya Inoue, Takuya Egawa, Tsutomu Kira, Munehiro Ogawa, Yasuhito Tanaka, Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara 634-8522, Japan
Manabu Akahane, Department of Public Health, Health Management and Policy, Nara Medical University, Faculty of Medicine, Kashihara, Nara 634-8521, Japan
Kenji Kawate, Departments of Artificial Joint and Regenerative Medicine for Bone and Cartilage, Nara Medical University, Kashihara, Nara 634-8522, Japan
Author contributions: Inagaki Y and Akahane M designed the study; Inagaki Y, Akahane M, Shimizu T, Inoue K, Egawa T and Kira T performed the study; Inagaki Y and Akahane M drafted the manuscript; Ogawa M, Kawate K and Tanaka Y critically reviewed the draft; all authors read and approved the final manuscript.
Institutional review board statement: This study was approved by the Institutional Review Board of Nara Medical University before beginning experiments.
Institutional animal care and use committee statement: All experimental protocols using animals were approved by the Animal Experimental Review Board of Nara Medical University before beginning experiments.
Conflict-of-interest statement: There is no conflict of interest regarding to this study.
Data sharing statement: None.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Yusuke Inagaki, MD, PhD, Assisstant Professor, Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan. yinagaki@naramed-u.ac.jp
Telephone: +81-744-223051 Fax: +81-744-256449
Received: January 26, 2017
Peer-review started: February 2, 2017
First decision: March 31, 2017
Revised: April 27, 2017
Accepted: June 6, 2017
Article in press: June 8, 2017
Published online: July 26, 2017
Core Tip

Core tip: Bone tissue engineering using marrow stromal cells (MSCs) is a promising method in regenerative medicine. Here, we have reported a scaffold-free transplantation technique using hypoxic-preconditioned osteogenic matrix cell sheets (OMCSs) derived from MSCs. We show that modifying the oxygen tension before implantation of OMCS composites led to an increased osteogenic capacity of rat bone MSCs.