Ting S, Lecina M, Chan YC, Tse HF, Reuveny S, Oh SK. Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells. World J Stem Cells 2013; 5(3): 86-97 [PMID: 23904910 DOI: 10.4252/wjsc.v5.i3.86]
Corresponding Author of This Article
Dr. Steve KW Oh, Principal Scientist, Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), 20 Biopolis Way No. 06-01, Singapore 138668, Singapore. steve_oh@bti.a-star.edu.sg
Research Domain of This Article
Developmental Biology
Article-Type of This Article
Original Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Sherwin Ting, Marti Lecina, Shaul Reuveny, Steve KW Oh, Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), Singapore 138668, Singapore
Yau-Chi Chan, Hung Fat Tse, Cardiology Division, Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, China
Author contributions: Ting S and Lecina M contributed to conception and design; Ting S, Lecina M, and Chan YC contributed to collection and assembly of data; Ting S, Reuveny S and Reuveny S contributed to manuscript writing; all authors contributed to data analysis and interpretation, and final approval of manuscript.
Supported by Science Technology and Research (A*STAR); Hong Hong Kong Research Grant Council Collaborative Research Fund (HKU8/CRF/09) and Theme-based Research Scheme (T12-705/11); Tse HT and Oh SKW contributed to financial support
Correspondence to: Dr. Steve KW Oh, Principal Scientist, Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), 20 Biopolis Way No. 06-01, Singapore 138668, Singapore. steve_oh@bti.a-star.edu.sg
Telephone: +65-6407-0855 Fax: +65-6478-9561
Received: March 26, 2013 Revised: June 3, 2013 Accepted: June 8, 2013 Published online: July 26, 2013 Processing time: 120 Days and 18 Hours
Core Tip
Core tip: Nutrient supplements were screened for improving cell survival during the cardiomyocyte differentiation process of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) in a serum-free medium based on the inhibition of p38 MAPK (SB media). Soy hydrolysate and bovine serum albumin supplementation was found to improve cell viability and therefore increased the yield of HES-3 cardiomyocytes by 2.6-fold over non-supplemented SB medium. The enhancing effect of this medium was demonstrated in an additional hESC line (H1) and Siu1-hiPSC cell line (15 and 9 fold respectively). The cardiomyocytes formed expressed canonical cardiac markers (sarcomeric α-actinin, myosin heavy chain and troponin-T) and demonstrated all three major phenotypes: nodal-, atrial- and ventricular-like.
