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©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Apr 26, 2025; 17(4): 101290
Published online Apr 26, 2025. doi: 10.4252/wjsc.v17.i4.101290
Published online Apr 26, 2025. doi: 10.4252/wjsc.v17.i4.101290
RNA interference-mediated osteoprotegerin silencing increases the receptor activator of nuclear factor-kappa B ligand/osteoprotegerin ratio and promotes osteoclastogenesis
Song-Guan Wei, Hui-Hong Chen, Liu-Rong Xie, Yuan Qin, Yu-Ying Mai, Lin-Hui Huang, Hong-Bing Liao, Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, College & Hospital of Stomatology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
Song-Guan Wei, Department of Stomatology, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou 545005, Guangxi Zhuang Autonomous Region, China
Author contributions: Wei SG and Chen HH designed the study and performed the experiments; Qin Y and Xie LR conducted the gene silencing and osteoclastogenesis assays; Mai YY and Huang LH contributed to sample preparation and co-immunoprecipitation assays; Liao HB supervised the project and drafted the manuscript. All authors reviewed and approved the final manuscript.
Supported by the National Natural Science Foundation of China, No. 82160192; and Guangxi Science and Technology Program, No. 2023AB23037.
Institutional animal care and use committee statement: All animal experiments were approved by the Animal Ethics Committee of Guangxi Medical University, No. 202111005.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: The data used to support the findings of this study are available from the corresponding author upon reasonable request.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hong-Bing Liao, PhD, Professor, Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, College & Hospital of Stomatology, Guangxi Medical University, No. 22 Shuangyong Road, Qingxiu District, Nanning 530021, Guangxi Zhuang Autonomous Region, China. hongbing_liao@gxmu.edu.cn
Received: September 10, 2024
Revised: December 13, 2024
Accepted: March 21, 2025
Published online: April 26, 2025
Processing time: 224 Days and 22.1 Hours
Revised: December 13, 2024
Accepted: March 21, 2025
Published online: April 26, 2025
Processing time: 224 Days and 22.1 Hours
Core Tip
Core Tip: This study reports, for the first time, that enhancing the receptor activator of nuclear factor-kappa B ligand/osteoprotegerin (RANKL/OPG) ratio through RNA interference promotes osteoclastogenesis. Our findings reveal a significant upregulation of RANKL mRNA levels after OPG gene silencing. The study demonstrates a significant downregulation of OPG mRNA and protein levels. The increase in the RANKL/OPG ratio significantly promotes osteoclastogenesis. This study provides a new theoretical basis and molecular targets for degrading bone scaffolds and bone tissue repair.