Basic Study
Copyright ©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Mar 26, 2025; 17(3): 100079
Published online Mar 26, 2025. doi: 10.4252/wjsc.v17.i3.100079
Regulation of lncRNA-ENST on Myc-mediated mitochondrial apoptosis in mesenchymal stem cells: In vitro evidence implicated for acute lung injury therapeutic potential
Ye-Zhou Shen, Guang-Ping Yang, Qi-Min Ma, Yu-Song Wang, Xin Wang
Ye-Zhou Shen, Qi-Min Ma, Yu-Song Wang, Department of Critical Care Medicine, Shanghai East Hospital, Tongji University, Shanghai 200120, China
Guang-Ping Yang, Xin Wang, Medical Center of Burn Plastic and Wound Repair, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
Co-first authors: Ye-Zhou Shen and Guang-Ping Yang.
Author contributions: Shen YZ and Yang GY contributed equally to the study design, data analysis, and manuscript preparation and are co-first authors of this manuscript; Shen YZ and Wang X were involved in the study design; Shen YZ and Yang GP mainly conducted the experiments and the statistical analyses, and wrote the manuscript; Ma QM and Wang YS assisted in data analysis. All authors have read the final manuscript and approved the submitted version.
Supported by the Peak Supporting Clinical Discipline of Shanghai Health Bureau, No. 2023ZDFC0104; and the National Key R&D Program of China, No. 2019YFA0110601.
Institutional review board statement: The study described in this manuscript involved the use of stem cell-derived extracellular vesicles (exosomes) in vitro, without the involvement of any human or animal subjects. As such, it did not require ethical review or approval from an Institutional Review Board or Institutional Ethics Committee. The research was conducted in compliance with all relevant guidelines and regulations for in vitro research, and no ethical concerns were raised in the context of this study.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: Technical appendix, statistical code, and dataset available upon reasonable request from the corresponding author at 18772407841@163.com.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Xin Wang, Medical Center of Burn Plastic and Wound Repair, The First Affiliated Hospital of Nanchang University, No. 17 Yongwai Zhengjie, Donghu District, Nanchang 330006, Jiangxi Province, China. 18772407841@163.com
Received: August 6, 2024
Revised: December 4, 2024
Accepted: February 5, 2025
Published online: March 26, 2025
Processing time: 226 Days and 17 Hours
Core Tip

Core Tip: In this research, we established an acute lung injury (ALI) model and manipulated long noncoding RNA (lncRNA)-ENST levels in bone marrow mesenchymal stem cells (BMSCs) using a lentiviral system. We discovered that lncRNA-ENST00000517482 is a pivotal regulator of BMSC apoptosis and autophagy in ALI. Modulating lncRNA-ENST00000517482 not only reduces apoptosis and induces autophagy in BMSCs but also enhances their viability, offering a novel approach to enhance ALI treatment efficacy via the miR-539/c-MYC axis.