Basic Study
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World J Stem Cells. Jan 26, 2025; 17(1): 98349
Published online Jan 26, 2025. doi: 10.4252/wjsc.v17.i1.98349
Bone marrow mesenchymal stem cells promote uterine healing by activating the PI3K/AKT pathway and modulating inflammation in rat models
Jing Yang, Jun Yuan, Yan-Qing Wen, Li Wu, Jiu-Jiang Liao, Hong-Bo Qi
Jing Yang, Jun Yuan, Yan-Qing Wen, Li Wu, Jiu-Jiang Liao, Hong-Bo Qi, Women and Children’s Hospital of Chongqing Medical University, Chongqing 401147, China
Jing Yang, Jun Yuan, Yan-Qing Wen, Jiu-Jiang Liao, Hong-Bo Qi, Chongqing Key Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing 400016, China
Jing Yang, Obstetrics and Gynecology, Guizhou Provincial People’s Hospital, Guiyang 557300, Guizhou Province, China
Hong-Bo Qi, Research Laboratory of Reproduction and Development of Chinese Ministry of Education, Chongqing Medical University, Chongqing 400016, China
Co-corresponding authors: Jiu-Jiang Liao and Hong-Bo Qi.
Author contributions: Yang J, Liao JJ, and Qi HB made substantial contributions to the conception and design of the study, drafting the article and critically revising it for important intellectual content; Yang J and Yuan J performed the experiments; Yang J, Wen YQ, and Wu L acquired and analyzed the data; All authors approved the final version for publication. Liao JJ and Qi HB contributed to the discussion of the data, funding acquisition and supervision; they are co-corresponding authors of this manuscript.
Supported by the National Natural Science Foundation of China, No. 82301919; China Postdoctoral Science Foundation, No. 2023M730441; Guizhou Provincial Science and Technology Projects, No. [2020]1Y149; Joint Funds of the National Natural Science Foundation of China, No. U21A20346; and the Key Research Program of Chongqing Science and Technology Bureau, No. CSTB2022TIAD-KPX0156.
Institutional animal care and use committee statement: This study and the experimental procedures were approved by the Guizhou Provincial People’s Hospital. All animal experiments were approved by the Animal Care and Use Committee of the Ethical Institution of the Guizhou Provincial People’s Hospital, ethics approval number: (2019)163.
Conflict-of-interest statement: The authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hong-Bo Qi, MD, PhD, Professor, Women and Children’s Hospital of Chongqing Medical University, No. 120 Longshan Road, Yubei District, Chongqing 401147, China. qihongbo@cqmu.edu.cn
Received: July 2, 2024
Revised: October 2, 2024
Accepted: December 10, 2024
Published online: January 26, 2025
Processing time: 202 Days and 2.2 Hours
Core Tip

Core Tip: We identified differentially genes in uterine wound tissues through transcriptome sequencing. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that regeneration and anti-inflammatory factors were upregulated in bone mesenchymal stem cell (BMSCs) groups and that the phosphoinositide 3-kinase/protein kinase B/mechanistic target of rapamycin pathway was highly enriched. This study also demonstrated that BMSCs significantly promoted the proliferation and migration of uterine smooth muscle cells (USMCs) and that USMCs enhanced the myogenic differentiation of BMSCs. In vivo and in vitro experiments demonstrate that BMSCs activate the phosphoinositide 3-kinase/protein kinase B pathway in wound tissues and USMCs.