Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Jun 26, 2024; 16(6): 656-669
Published online Jun 26, 2024. doi: 10.4252/wjsc.v16.i6.656
RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation
Daniel B Ferreira, Leticia M Gasparoni, Cristiane F Bronzeri, Katiucia B S Paiva
Daniel B Ferreira, Leticia M Gasparoni, Cristiane F Bronzeri, Katiucia B S Paiva, Laboratory of Extracellular Matrix Biology and Cellular Interaction, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, São Paulo, Brazil
Author contributions: Gasparoni LM and Bronzeri CF performed the experiments and acquired the data; Ferreira DB and Paiva KBS analysed the data and wrote the manuscript; Paiva KBS designed and coordinated the study; and all authors approved the final version of the article.
Supported by São Paulo Research Foundation (FAPESP), No. 2010/08918-9 and 2020/11564-6; the KBSP Young Investigator Fellowship, No. 2011/00204-0; the DBF Fellowship, No. 2019/27492-7; the LMG Fellowship, No. 2014/01395-1; and the CFB Fellowship, No. 2014/14278-3.
Institutional review board statement: The study was reviewed and approved by the Human Research Ethics Committee of the Institute of Biomedical Sciences and University Hospital, both University of São Paulo (CAAE: 51097315.7.0000.5467 and CAAE: 51097315.7.3001.0076).
Conflict-of-interest statement: All authors have no conflicts of interest to declare.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Katiucia B S Paiva, BSc, PhD, Assistant Professor, Laboratory of Extracellular Matrix Biology and Cellular Interaction, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes n. 2.415 (ICB3 - laboratório 111), São Paulo 05508-000, São Paulo, Brazil. katipaiva@usp.br
Received: November 23, 2023
Revised: January 23, 2024
Accepted: April 12, 2024
Published online: June 26, 2024
Processing time: 214 Days and 21.2 Hours
Core Tip

Core Tip: Detecting the best reference genes (RGs) under specific conditions is a good practice to improve the understanding of gene expression. Stem cells have been largely studied during commitment to particular cell lineages for many applications, such as tissue engineering. In this way, dental pulp stem cells (DPSCs) are promising for craniofacial reconstruction. For the first time, we show that the best pair of RGs for the osteogenic differentiation of human DPSCs are ribosomal protein, large, P0/TATA-binding protein by quantitative real-time polymerase chain reaction through the four algorithms (ΔCt comparative method, geNorm, BestKeeper, and NormFinder) and ranked by RefFinder.