Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. May 26, 2024; 16(5): 575-590
Published online May 26, 2024. doi: 10.4252/wjsc.v16.i5.575
Hepatocyte growth factor enhances the ability of dental pulp stem cells to ameliorate atherosclerosis in apolipoprotein E-knockout mice
Han Duan, Ning Tao, Lin Lv, Kai-Xin Yan, Yong-Gang You, Zhuang Mao, Chang-Yao Wang, Xue Li, Jia-Yan Jin, Chu-Tse Wu, Hua Wang
Han Duan, Chang-Yao Wang, Hua Wang, School of Life Sciences, Hebei University, Baoding 071002, Hebei Province, China
Ning Tao, Lin Lv, Zhuang Mao, Xue Li, Chu-Tse Wu, Hua Wang, Beijing Institute of Radiation Medicine, Beijing 100850, China
Kai-Xin Yan, Department of Cardiology, The Sixth Medical Centre, Chinese People’s Liberation Army General Hospital, Beijing 100037, China
Yong-Gang You, Department of Orthopaedics, The Fourth Medical Centre, Chinese People’s Liberation Army General Hospital, Beijing 100853, China
Jia-Yan Jin, Third Cadet Regiment, School of Basic Medical Science, Air Force Medical University, Xi’an 710032, Shaanxi Province, China
Co-first authors: Han Duan and Ning Tao.
Author contributions: Duan H and Tao N contributed equally to this study. These contributions include study design, acquisition and analysis of experimental data, and writing the actual manuscript. Wang H contributed to the conceptualization and writing - review & editing; Duan H, Tao N, and Lv L were involved in the data curation; Duan H, Tao N, Lv L, Yan KX, You YG, Mao Z, Wang CY, Li X, and Jin JY participated in the formal analysis; Duan H and Tao N contributed to the methodology of this manuscript; Wu CT was major in the supervision of this article; Duan H wrote original draft.
Institutional animal care and use committee statement: All procedures that involved animals were approved by the Institutional Animal Care and Use Committee of Beijing Institute of Radiation Medicine (IACUC-DWZX-2021-714).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hua Wang, PhD, Beijing Institute of Radiation Medicine, No. 27 Taiping Road, Beijing 100850, China. 18511712135@163.com
Received: January 18, 2024
Revised: March 18, 2024
Accepted: April 9, 2024
Published online: May 26, 2024
Processing time: 127 Days and 7.6 Hours
Core Tip

Core Tip: In this study, we found that dental pulp stem cells (DPSCs) treatment reduced atherosclerotic plaque formation in apolipoprotein E-knockout (ApoE-/-) mice fed a high-fat diet and that Ad-hepatocyte growth factor (HGF) modified DPSCs (DPSC-HGF) treatment was more effective than Ad-Null modified DPSCs (DPSC-Null) treatment, which depended on the reduced expression of aortic endothelial cell adhesion molecule and inflammatory macrophages. In addition, DPSC-HGF had a greater inhibitory effect on the nuclear factor-κB signaling pathway in RAW264.7 cells and HAOECs cells under inflammatory stimulation than DPSC-Null. Taken together, these data suggest for the first time that DPSCs treatment can ameliorate AS in ApoE-/- mice and that DPSC-HGF has more impressive therapeutic potential than DPSC-Null and might be a new therapy for AS patients in the future.