Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Apr 26, 2024; 16(4): 434-443
Published online Apr 26, 2024. doi: 10.4252/wjsc.v16.i4.434
Effects of high glucose and severe hypoxia on the biological behavior of mesenchymal stem cells at various passages
Fatimah Almahasneh, Ejlal Abu-El-Rub, Ramada R Khasawneh, Rawan Almazari
Fatimah Almahasneh, Ejlal Abu-El-Rub, Ramada R Khasawneh, Rawan Almazari, Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid 21163, Jordan
Author contributions: Abu-El-Rub E conceptualized the study; Almahasneh F, Abu-El-Rub E, Khasawneh RR, and Almazari R carried out the experiments; Almahasneh F and Abu-El-Rub E analyzed the data, revised and formatted the content of the manuscript and verified spelling, punctuation and grammatical errors; Almahasneh F drafted and wrote the manuscript; and all authors have read and approve the final manuscript.
Supported by the Deanship of Scientific Research, Yarmouk University, Jordan, No. 73/2022.
Institutional review board statement: Institutional review board approval was not needed for this study because the human cell lines used for this study were commercially purchased.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Ejlal Abu-El-Rub, PharmD, PhD, Assistant Professor, Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid-Shafiq Irshidat Street, Irbid 21163, Jordan. ejlal.abuelrub@yu.edu.jo
Received: December 30, 2023
Peer-review started: December 30, 2023
First decision: January 23, 2024
Revised: February 5, 2024
Accepted: March 18, 2024
Article in press: March 18, 2024
Published online: April 26, 2024
Processing time: 116 Days and 8 Hours
Core Tip

Core Tip: Mesenchymal stem cells (MSCs) are increasingly being used for the treatment of various diseases due to their immunomodulatory and regenerative properties, but serial passages needed for MSCs expansion and stress factors found in the diseased tissues may compromise their therapeutic potential. Investigating the effects of stress factors (high glucose and severe hypoxia) on the biological characteristics of MSCs at different passages will help optimize the clinical uses of MSCs and their expected outcomes.