Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Feb 26, 2024; 16(2): 163-175
Published online Feb 26, 2024. doi: 10.4252/wjsc.v16.i2.163
Effects of different concentrations of nicotinamide on hematopoietic stem cells cultured in vitro
Yan Ren, Yan-Ni Cui, Hong-Wei Wang
Yan Ren, Yan-Ni Cui, The Second Clinical Medical College, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
Yan Ren, Yan-Ni Cui, Hong-Wei Wang, Department of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
Hong-Wei Wang, Department of Hematology, Key Laboratory of Molecular Diagnosis and Treatment of Blood Diseases in Shanxi Province, Taiyuan 030001, Shanxi Province, China
Co-first authors: Yan Ren and Yan-Ni Cui.
Author contributions: Ren Y and Cui YN designed and coordinated the study; Ren Y performed experiments and wrote the manuscript; Cui YN acquired and analyzed the data; Wang HW contributed to ideas, supervision, review and editing; and all authors approved the final version of the article.
Supported by the Science and Technology Department of Shanxi Province, No. YDZJSX2021B009; Health Commission of Shanxi Province, No. 2021XM07; and Shanxi Provincial Department of Education, No. 2023KY380.
Institutional review board statement: This study was approved by the Second Hospital of Shanxi Medical University (Approval No. 20230055).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hong-Wei Wang, PhD, Professor, Department of Hematology, The Second Hospital of Shanxi Medical University, No. 382 Wuyi Road, Taiyuan 030001, Shanxi Province, China. wanghw68@hotmail.com
Received: October 18, 2023
Peer-review started: October 18, 2023
First decision: December 2, 2023
Revised: December 14, 2023
Accepted: January 12, 2024
Article in press: January 12, 2024
Published online: February 26, 2024
Processing time: 130 Days and 18.6 Hours
Core Tip

Core tip: This study reveals the dominant subgroups of hematopoietic stem cells (HSCs) and molecular mechanisms underlying the effects of different nicotinamide (NAM) concentrations. Activation or inhibition of sirtuin 1 (SIRT1) is determined by the concentration of NAM. High concentrations inhibit SIRT1 but are not conducive to self-renewal of HSCs, whereas low concentrations balance HSC proliferation and differentiation by regulating the SIRT1–HIF1A pathway and reactive oxygen species production, effectively promoting in vitro expansion of the stem cells. These findings could allow adjustment of NAM concentrations according to expansion needs and may help predict small molecules that synergistically promote expansion with NAM.