Basic Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Jun 26, 2023; 15(6): 589-606
Published online Jun 26, 2023. doi: 10.4252/wjsc.v15.i6.589
Single cell RNA sequencing reveals mesenchymal heterogeneity and critical functions of Cd271 in tooth development
Yan-Yan Zhang, Feng Li, Xiao-Ke Zeng, Yan-Hui Zou, Bing-Bing Zhu, Jia-Jia Ye, Yun-Xiao Zhang, Qiu Jin, Xin Nie
Yan-Yan Zhang, Feng Li, Xiao-Ke Zeng, Yan-Hui Zou, Bing-Bing Zhu, Jia-Jia Ye, Yun-Xiao Zhang, Qiu Jin, Xin Nie, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China
Author contributions: Zhang YY conceived experiments, interpreted data and wrote a manuscript; Li F, Zeng XK and Zou YH performed the experiment; Zhu BB, Ye JJ, Zhang YX, and Jin Q provided a critical insight into this study; Nie X supervised this study; All authors read and approved the final version for submission.
Supported by National Natural Science Foundation of China (General Program), No. 31870971; and Medical Health Science and Technology Project of Zhejiang Province, No. 2023KY155.
Institutional animal care and use committee statement: All animal experiments were performed according to the protocols approved by the Medical Ethics Committee of Wenzhou Medical University, No. wydw2019-0224.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The datasets analyzed for this study can be found in the NCBI SRA: 506PRJNA891206. https://www.ncbi.nlm.nih.gov/sra/PRJNA891206.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Xin Nie, PhD, Professor, School & Hospital of Stomatology, Wenzhou Medical University, Xueyuan West Road, Lucheng District, Wenzhou 325027, Zhejiang Province, China. dr.xinnie@qq.com
Received: February 9, 2023
Peer-review started: February 9, 2023
First decision: April 10, 2023
Revised: April 18, 2023
Accepted: May 5, 2023
Article in press: May 5, 2023
Published online: June 26, 2023
Processing time: 137 Days and 8.9 Hours
Core Tip

Core Tip: Our study reveals the following findings: (1) High cellular heterogeneity and molecular details; (2) Significant functional and signaling differences between cell types; (3) Novel subclusters of mesenchymal stem cells; and (4) Crucial cell-cell interactions of mesenchymal subpopulations. We provided new insights into the biological features of mesenchymal stem cells at the single cell level. Our findings contribute to thorough exploration of the mechanism of Cd271 in regulating odontogenesis and osteogenesis which add to the theory of tooth development.