Basic Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Mar 26, 2023; 15(3): 90-104
Published online Mar 26, 2023. doi: 10.4252/wjsc.v15.i3.90
Mammalian Ste20-like kinase 1 inhibition as a cellular mediator of anoikis in mouse bone marrow mesenchymal stem cells
Tao Zhang, Qian Zhang, Wan-Cheng Yu
Tao Zhang, Qian Zhang, Wan-Cheng Yu, Department of Cardiovascular Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250062, Shandong Province, China
Author contributions: Yu WC contributed to the conception and design and manuscript writing; Zhang T contributed to conception and design, collection and assembly of data, data analysis and interpretation. Yu WC, Zhang T, Zhang Q performed the experiments; All authors participated in discussing, revising the manuscript, and approving the final manuscript; All authors have read and approve the final manuscript.
Supported by Natural Science Foundation of Shandong Province, China, No. ZR2020MH014, No. ZR2021QH179 and No. ZR2021MH182.
Institutional animal care and use committee statement: Ethical approval was obtain from the Institutional Animal Care and Use Committee of Shandong Provincial Hospital Affiliated to Shandong First Medical University. Experimental procedures were carried out according to the internationally accepted norms and principles under protocol (Approval No. 2020-333).
Conflict-of-interest statement: The authors declare that they have no conflicts of interest with the contents of this article.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Wan-Cheng Yu, Doctor, MD, Surgeon, Department of Cardiovascular Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No. 324 Jingwu Weiqi Road, Jinan 250062, Shandong Province, China. yuwancheng123@126.com
Received: June 30, 2022
Peer-review started: June 30, 2022
First decision: December 30, 2022
Revised: January 6, 2023
Accepted: February 16, 2023
Article in press: February 16, 2023
Published online: March 26, 2023
Core Tip

Core Tip: In isolated mouse bone marrow mesenchymal stem cell (mBMSCs), Mammalian sterile 20-like kinase 1 (Mst1) inhibition could ameliorate not only autophagy formation but also upregulate integrin (ITG) α5β1 expression (but not ITGα4, ITGαv, or ITGβ3). In addition, Mst1 inhibition-induced autophagy could scavenge the excessive production of ITGα5β1-triggered ROS. Therefore, Mst1 inhibition-based infusion may improve the survival of MSCs, thereby serving as an ideal candidate for clinical transplantation in pulmonary arterial hypertension.