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©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Sep 26, 2022; 14(9): 700-713
Published online Sep 26, 2022. doi: 10.4252/wjsc.v14.i9.700
Published online Sep 26, 2022. doi: 10.4252/wjsc.v14.i9.700
Overexpression of GATA binding protein 4 and myocyte enhancer factor 2C induces differentiation of mesenchymal stem cells into cardiac-like cells
Syeda Saima Razzaq, Kanwal Haneef, Dr. Zafar H. Zaidi Center for Proteomics, University of Karachi, Karachi 75270, Pakistan
Irfan Khan, Asmat Salim, Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan
Nadia Naeem, Dow Research Institute of Biotechnology & Biomedical Sciences (DRIBBS), Dow University of Health Sciences (DUHS), Ojha Campus, Karachi 75200, Pakistan
Sumreen Begum, Stem Cells Research Laboratory (SCRL), Sindh Institute of Urology and Transplantation (SIUT), Karachi 74200, Pakistan
Author contributions: Razzaq SS performed the experiments, did data analysis, and wrote the first draft of the manuscript; Khan I co-supervised the research study and assisted in transfection studies; Naeem N assisted in qPCR; Begum S assisted in immunocytochemistry; Salim A assisted in all in vitro studies; Haneef K conceived the idea, supervised the research study, and finalized the manuscript.
Supported by the Higher Education Commission (HEC) , Pakistan Scholarship for Ph.D. Studies to Razzaq SS, No. 520-148390-2BS6-011.
Institutional review board statement: The study was reviewed and approved by the institutional bioethical committee of University of Karachi (No. ICB KU-92/2020).
Conflict-of-interest statement: The authors confirm that this article has no conflict of interest to report.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Kanwal Haneef, PhD, Assistant Professor, Dr. Zafar H. Zaidi Center for Proteomics, University of Karachi, University Road, Karachi 75270, Pakistan.
Received: March 28, 2022
Peer-review started: March 28, 2022
First decision: June 11, 2022
Revised: June 20, 2022
Accepted: August 30, 2022
Article in press: August 30, 2022
Published online: September 26, 2022
Processing time: 179 Days and 1.2 Hours
Peer-review started: March 28, 2022
First decision: June 11, 2022
Revised: June 20, 2022
Accepted: August 30, 2022
Article in press: August 30, 2022
Published online: September 26, 2022
Processing time: 179 Days and 1.2 Hours
Core Tip
Core Tip: Transcription factors have great potential to direct cell fate decisions during embryonic development. In this study, we investigated the overexpression of cardiac transcription factors in human umbilical cord mesenchymal stem cells to enhance their differentiation into cardiac-like cells. The synergistic effect of GATA binding protein 4 and myocyte enhancer factor 2C transcription factors increased the expression of cardiac genes and proteins. The results of this study will aid in the development of new therapeutic strategies aimed at curing heart diseases.