Basic Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Aug 26, 2022; 14(8): 616-632
Published online Aug 26, 2022. doi: 10.4252/wjsc.v14.i8.616
Changes of cell membrane fluidity for mesenchymal stem cell spheroids on biomaterial surfaces
Chui-Wei Wong, Hao-Wei Han, Shan-hui Hsu
Chui-Wei Wong, Hao-Wei Han, Shan-hui Hsu, National Taiwan University, Institute of Polymer Science and Engineering, Taipei 10617, Taiwan
Shan-hui Hsu, National Health Research Institutes, Institute of Cellular and System Medicine, Miaoli 350, Taiwan
Shan-hui Hsu, National Taiwan University, Research and Development Center for Medical Devices, Taipei 10617, Taiwan
Author contributions: Wong CW and Han HW designed and coordinated the study, performed the experiments, acquired and interpreted the data, and wrote the original draft; Hsu Sh contributed to the conceptualization, investigation, review, editing and supervision; All authors approved the final version of the article.
Supported by National Taiwan University Core Consortium, No. NTU-CC-110L892501; and Ministry of Science and Technology, No. MOST 110-2218-E-002-037.
Institutional review board statement: The study does not meet the definition of a human subject and does not require an institutional review board statement review at National Taiwan University.
Institutional animal care and use committee statement: No animal study in the manuscript.
Conflict-of-interest statement: The authors have no conflicts of interest to declare.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Shan-hui Hsu, PhD, Professor, National Taiwan University, Institute of Polymer Science and Engineering, No. 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan. shhsu@ntu.edu.tw
Received: March 5, 2022
Peer-review started: March 5, 2022
First decision: April 19, 2022
Revised: May 2, 2022
Accepted: July 8, 2022
Article in press: July 8, 2022
Published online: August 26, 2022
Processing time: 174 Days and 3.5 Hours
Core Tip

Core Tip: The fluidity of the cell membrane is an important physical property for the regulation of cell behavior. To date, the changes in cell membrane fluidity are not fully understood in spheroid-forming cells. In this study, various substrates were utilized to grow mesenchymal stem cells and to form homotypic or heterotypic cellular spheroids on the substrates. During the spheroid-forming process, vesicle-like bubbles were randomly generated on the cell membrane. The evidence suggests that the physiological alterations of cells after they form cellular spheroids may be associated with changes in membrane fluidity during the spheroid-forming process.