Review
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Jun 26, 2022; 14(6): 393-419
Published online Jun 26, 2022. doi: 10.4252/wjsc.v14.i6.393
Application and prospects of high-throughput screening for in vitro neurogenesis
Shu-Yuan Zhang, Juan Zhao, Jun-Jun Ni, Hui Li, Zhen-Zhen Quan, Hong Qing
Shu-Yuan Zhang, Jun-Jun Ni, Hui Li, Zhen-Zhen Quan, Hong Qing, Key Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, Department of Biology, School of Life Science, Beijing Institute of Technology, Beijing 100081, China
Juan Zhao, Aerospace Medical Center, Aerospace Center Hospital, Beijing 100049, China
Author contributions: The review was conceived and designed by Qing H, Zhao J and Zhang SY; The study was drafted by Zhang SY; The review was revised by Qing H, Zhao J and Zhang SY; The review was discussed by Qing H, Zhao J, Ni JJ, Quan ZZ, Li H and Zhang SY.
Supported by National Natural Science Foundation of China, No. 81870844, No. 82001167 and No. 82101394.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hong Qing, MD, PhD, Professor, Key Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, Department of Biology, School of Life Science, Beijing Institute of Technology, No. 5 Yard, Zhong Guan Cun South Street, Haidian District, Beijing 100081, China. hqing@bit.edu.cn
Received: December 10, 2021
Peer-review started: December 10, 2021
First decision: March 13, 2022
Revised: April 7, 2022
Accepted: May 28, 2022
Article in press: May 28, 2022
Published online: June 26, 2022
Processing time: 195 Days and 19.7 Hours
Core Tip

Core Tip: High-throughput screening (HTS) is a promising technology that can screen out targets from thousands of candidates. Here, we review the evidence that HTS could be beneficial in neurogenesis methods in various ways: The HTS method can screen out specific genes that induce neural induction, small molecules that facilitate neural differentiation, and three-dimensional microenvironments that could better modulate the microenvironments in vivo. We also focus on the application and prospects of HTS in in vitro neurogenesis, as organoid-based and microfluidic platforms are needed for future research.