Basic Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Mar 26, 2022; 14(3): 231-244
Published online Mar 26, 2022. doi: 10.4252/wjsc.v14.i3.231
In vitro induced pluripotency from urine-derived cells in porcine
Kaiana Recchia, Lucas Simões Machado, Ramon Cesar Botigelli, Naira Caroline Godoy Pieri, Gabriela Barbosa, Raquel Vasconcelos Guimarães de Castro, Mariana Groke Marques, Laís Vicari de Figueiredo Pessôa, Paulo Fantinato Neto, Flávio Vieira Meirelles, Aline Fernanda de Souza, Simone Maria Massami Kitamura Martins, Fabiana Fernandes Bressan
Kaiana Recchia, Lucas Simões Machado, Flávio Vieira Meirelles, Fabiana Fernandes Bressan, Department of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Pirassununga 13635-000, São Paulo, Brazil
Ramon Cesar Botigelli, Department of Pharmacology and Biotechnology, Institute of Bioscience, São Paulo State University, Botucatu 18618-689, São Paulo, Brazil
Naira Caroline Godoy Pieri, Gabriela Barbosa, Raquel Vasconcelos Guimarães de Castro, Laís Vicari de Figueiredo Pessôa, Paulo Fantinato Neto, Flávio Vieira Meirelles, Aline Fernanda de Souza, Fabiana Fernandes Bressan, Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga 13635-000, São Paulo, Brazil
Mariana Groke Marques, Embrapa Suínos e Aves, Empresa Brasileira de Pesquisa Agropecuária, Concordia 89715-899, Santa Catarina, Brazil
Simone Maria Massami Kitamura Martins, Department of Animal Sciences, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga 13635-000, São Paulo, Brazil
Author contributions: Recchia K and Bressan FF conceptualized the experiments and wrote the manuscript; Recchia K, Machado LS, Barbosa G, Marques MG and Martins SMMK collected and processed samples; Recchia K, Botigelli RC, Pieri NCG, de Castro RVG, Meirelles FV, de Souza AF, Pessôa LVF, Fantinato Neto P and Bressan FF collected data and interpreted the collected data; all authors revised the manuscript.
Supported by the São Paulo Research Foundation (FAPESP) - Brazil (financial support grants #2015/26818-5, #2013/08135-2, #2019/02811-2); Coordination of Superior Level Staff Improvement (CAPES 23038.006964/2014-43 and financial code 001) and National Council for Scientific and Technological Development (CNPq 433133/2018-0).
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board at Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo.
Institutional animal care and use committee statement: All procedures were performed following the National Council for Control of Animal Experimentation (CONCEA) rules and were approved by the Ethics Committee on Animal Experimentation of the Faculty of Animal Science and Food Engineering and Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo (protocols 6372070119 and 7051150717).
Conflict-of-interest statement: The authors declare that there are no conflicts of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Fabiana Fernandes Bressan, DVM, Professor, Department of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Av Duque de Caxias Norte 225, Pirassununga 13635-000, São Paulo, Brazil. fabianabressan@usp.br
Received: August 23, 2021
Peer-review started: August 23, 2021
First decision: October 3, 2021
Revised: October 11, 2021
Accepted: February 15, 2022
Article in press: February 15, 2022
Published online: March 26, 2022
Processing time: 213 Days and 19.5 Hours
Core Tip

Core Tip: The porcine induced pluripotent stem cells (piPSCs) derived from urine derived cells (UDCs) may facilitate their routine and large-scale use by avoiding injury or stress during collection for autologous purposes. However, the precise reprogramming process and characterization is not fully elucidated in other species than murine or human. The generation of piPSCs from UDCs can contribute as a biomedical model for regenerative and translational medicine, as well as for animal production and to elucidate the reprogramming process in porcine, a large animal model.