Basic Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Dec 26, 2022; 14(12): 839-850
Published online Dec 26, 2022. doi: 10.4252/wjsc.v14.i12.839
Optimal concentration of mesenchymal stem cells for fracture healing in a rat model with long bone fracture
Myung-Seo Kim, Hyun-Ju Chung, Kang-Il Kim
Myung-Seo Kim, Kang-Il Kim, Department of Orthopaedic Surgery, School of Medicine, Kyung Hee University and Kyung Hee University Hospital at Gangdong, Seoul 05278, South Korea
Hyun-Ju Chung, Department of Core Research Laboratory, Clinical Research Institute, Kyung Hee University Hospital at Gangdong, Seoul 05278, South Korea
Author contributions: Kim MS collected and assembled the data and performed data analysis and interpretation, manuscript writing; Chung HJ analyzed the data and performed interpretation; Kim KI provided the study material and designed the research study and performed final approval of manuscript; and all authors have read and approve the final manuscript.
Supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea, No. HI20C1405.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board at Kyung Hee University Hospital at Gangdong (KHNMC 2022-11-017).
Institutional animal care and use committee statement: All procedures and treatments involving animals in this study followed the requirements of the Institutional Animal Care and Use Committee of the Clinical Research Institute, and the final approval was obtained from the ethics committee of Kyung Hee University Hospital at Gangdong (KHNMC AP 2020-018).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: Data underlying this article are available in the article and in its online supplementary material.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Kang-Il Kim, MD, PhD, Professor, Department of Orthopaedic Surgery, School of Medicine, Kyung Hee University and Kyung Hee University Hospital at Gangdong, 892, Dongnam-ro, Gangdong-gu, Seoul 05278, South Korea. khuknee@gmail.com
Received: September 27, 2022
Peer-review started: September 27, 2022
First decision: October 10, 2022
Revised: October 30, 2022
Accepted: December 7, 2022
Article in press: December 7, 2022
Published online: December 26, 2022
Processing time: 84 Days and 18.3 Hours
Core Tip

Core Tip: This study focused on the optimal concentration of mesenchymal stem cells (MSCs) that affect fracture healing in a rat model of long bone shaft fracture. Factors related to the homing effect of MSCs, osteogenesis and angiogenesis were analyzed by in vivo (radiographic and histologic evaluation) as well as in vitro (reverse transcriptase-polymerase chain reaction and western blot analysis). Among the various concentrations used, the administration of at least 5.0 × 106 MSCs was optimal to promote the therapeutic effect on fracture healing.