Erol OD, Pervin B, Seker ME, Aerts-Kaya F. Effects of storage media, supplements and cryopreservation methods on quality of stem cells. World J Stem Cells 2021; 13(9): 1197-1214 [PMID: 34630858 DOI: 10.4252/wjsc.v13.i9.1197]
Corresponding Author of This Article
Fatima Aerts-Kaya, MD, PhD, Associate Professor, Department of Stem Cell Sciences, Hacettepe University Graduate School of Health Sciences, Ankara 06100, Turkey. fatimaaerts@yahoo.com
Research Domain of This Article
Cell Biology
Article-Type of This Article
Review
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Stem Cells. Sep 26, 2021; 13(9): 1197-1214 Published online Sep 26, 2021. doi: 10.4252/wjsc.v13.i9.1197
Effects of storage media, supplements and cryopreservation methods on quality of stem cells
Ozgur Dogus Erol, Burcu Pervin, Mehmet Emin Seker, Fatima Aerts-Kaya
Ozgur Dogus Erol, Burcu Pervin, Mehmet Emin Seker, Fatima Aerts-Kaya, Department of Stem Cell Sciences, Hacettepe University Graduate School of Health Sciences, Ankara 06100, Turkey
Ozgur Dogus Erol, Burcu Pervin, Mehmet Emin Seker, Fatima Aerts-Kaya, Center for Stem Cell Research and Development, Hacettepe University, Ankara 06100, Turkey
Author contributions: Erol OD, Pervin B and Seker ME drafted the first version of the article and contributed equally to this work; Aerts-Kaya F designed the manuscript, reviewed the contents and wrote the final version of the article to be published; All authors have read and approved the final manuscript.
Supported bythe Scientific and Technological Research Council of Turkey (TÜBİTAK), No. 118S738 and No. 219S675.
Conflict-of-interest statement: The authors declare no conflicts of interest.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Fatima Aerts-Kaya, MD, PhD, Associate Professor, Department of Stem Cell Sciences, Hacettepe University Graduate School of Health Sciences, Ankara 06100, Turkey. fatimaaerts@yahoo.com
Received: February 27, 2021 Peer-review started: February 27, 2021 First decision: April 20, 2021 Revised: April 21, 2021 Accepted: August 24, 2021 Article in press: August 24, 2021 Published online: September 26, 2021 Processing time: 202 Days and 10.7 Hours
Core Tip
Core Tip: The manuscript is an overview of current cryopreservation protocols used for cold storage of hematopoietic stem cells, mesenchymal stem cells and induced pluripotent stem cells. Although dimethyl sulfoxide (DMSO) is commonly used in cryopreservation of cell lines, primary cells and stem cells, the use of DMSO has been associated with certain toxicity, both directly on the cells, as well as upon infusion with the stem cell product. As a result of this many groups have undertaken efforts to find suitable replacements for DMSO that are equally potent but less toxic. In this review, we summarize the current status quo of stem cell freezing protocols and we describe the most commonly used cryoprotective agents and their effects on stem cells and stem cell function.
